The aim of our work was to find the positivity rate of antibodrrelian antibodies(IgG) in wild-living rodents in a locality situated in north Moravia, Czech Republic. Results of a surveyfor heart rinses (172) and sera (2) antibodies to Borrelia burgdorferi sensu lato (s.l.) from 6 speciesof 174 wild rodents from the northern part of the Czech Republic are presented. Samples were obtainedin 2001-2002 at one locality (Studenka, 49 degrees 44', 18 degrees 05'). Host samples included yellow-neckedmouse (Apodemus flavicollis, n = 106), wood mouse (A. sylvaticus, n = 170) and striped field mouse (A.agrarius, n = 13) from Muridae and bank vole (Clethrionomys glareolus, n = 3) and common/field vole (Microtussp., n = 5) from Microtidae families. An indirect enzyme linked immunosorbent assay (ELISA) antibodytest was used for testing heart rinses. Goat immunoglobulins against mouse were used as a conjugate.Antibodies to B. burgdorferi s.l. were found in all species. The highest positivity rate (58.8 % ) wasrecorded for wood mouse (58.8 % ), bank vole (45.5 % ), and yellow-necked mouse (44.3 % ). Mean positivityrate for both years of collection was 43.7 %, mean annual positivity rate was 60.6 % in 2001, and thevalue of 21.3 % in 2002 appeared significantly different. Three times as many of the Muridae as of thefamily Microtidae were caught and the actual number of seropositive Muridae was not significantly higherthan Microtidae. Positivity of compared males and females was not significantly different. Results indicatethat spirochaetes were widely represented in the northern part of the Czech Republic.