Online first
Current issue
Archive
Special Issues
About the Journal
Publication Ethics
Anti-Plagiarism system
Instructions for Authors
Instructions for Reviewers
Editorial Board
Editorial Office
Contact
Reviewers
All Reviewers
2023
2022
2021
2020
2019
2018
2017
2016
General Data Protection Regulation (RODO)
RESEARCH PAPER
The evaluation of enamel matrix derivative on subgingival microbial environment in non-surgical periodontal therapy
1
Department of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, Poland
2
Department of Medical Microbiology, Poznan University of Medical Sciences, Poland
3
Chair of Social Medicine, Poznan University of Medical Sciences, Poland
Corresponding author
Ann Agric Environ Med. 2013;20(3):431-435
KEYWORDS
ABSTRACT
Introduction and objective:
Recent in vitro studies indicate that enamel matrix derivative (EMD) could modulate the growth of periodontopathogens. The aim of the presented study was an analysis of the influence of EMD on the presence of Porphyromonas and Prevotella bacteria in the periodontal pockets of patients with chronic periodontitis in non-surgical periodontal therapy.
Material and Methods:
The studies were conducted on 20 patients. The condition of the periodontium was evaluated by clinical indexes: API. SBI. PD. CAL before and 3 months after the therapy in two selected quadrants. The material was collected for investigation. The periodontopathogens were cultured and identified. Two days after EMD-scaling root planing (SRP) was applied into the pockets.
Results:
In the group of patients under investigation before the EMD application the presence of P. gingivalis was found in 6 patients and P. intermedia in 8 patients. After root planing and EMD application no periodontopathogens were identified in those patients either in the periodontal pockets treated with EMD or in the periodontal pockets free from EMD (control). In the statistical analysis of changes in clinical indexes, the application of SRP and SRP combined with EMD was proved to significantly influence the improvement of the clinical state. However, no significant differences between the individual parameters were found in either group.
Conclusions:
The SRP is an effective method of limiting the development of periopathogens in periodontal pockets. The non-surgical therapy with EMD does not change the clinical parameters significantly, compared with the SRP. Simultaneously, the application of EMD inhibits the development of periopathogens, such as Porphyromonas gingivalis and Prevotella.
Recent in vitro studies indicate that enamel matrix derivative (EMD) could modulate the growth of periodontopathogens. The aim of the presented study was an analysis of the influence of EMD on the presence of Porphyromonas and Prevotella bacteria in the periodontal pockets of patients with chronic periodontitis in non-surgical periodontal therapy.
Material and Methods:
The studies were conducted on 20 patients. The condition of the periodontium was evaluated by clinical indexes: API. SBI. PD. CAL before and 3 months after the therapy in two selected quadrants. The material was collected for investigation. The periodontopathogens were cultured and identified. Two days after EMD-scaling root planing (SRP) was applied into the pockets.
Results:
In the group of patients under investigation before the EMD application the presence of P. gingivalis was found in 6 patients and P. intermedia in 8 patients. After root planing and EMD application no periodontopathogens were identified in those patients either in the periodontal pockets treated with EMD or in the periodontal pockets free from EMD (control). In the statistical analysis of changes in clinical indexes, the application of SRP and SRP combined with EMD was proved to significantly influence the improvement of the clinical state. However, no significant differences between the individual parameters were found in either group.
Conclusions:
The SRP is an effective method of limiting the development of periopathogens in periodontal pockets. The non-surgical therapy with EMD does not change the clinical parameters significantly, compared with the SRP. Simultaneously, the application of EMD inhibits the development of periopathogens, such as Porphyromonas gingivalis and Prevotella.
REFERENCES (35)
1.
Linde J, Lang NP, Karring T. Clinical Periodontology and Implant Dentistry. Blackwell Munksgaard 2008.
2.
Paster B, Olsen I, Aas JA, Dewhirst FE. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol.2000 2006; 42: 80–87.
3.
Kolenbrander PE, Parrish KD, Andersen RN, Greenberg EP. Intergeneric coaggregation of oral Treponema spp. with Fusobacterium spp. and intrageneric coaggregation among Fusobacterium spp. Infect Immunol. 1995; 63: 4584–4588.
4.
Listgarten MA. Structure of microbial flora associated with periodontal health and disease in man. A light and electron microscopic study. J Periodontol. 1976; 47: 1–18.
6.
Petersilka GJ, Ehmke B, Flemming TH. Antimicrobial effects of mechanical debridement. Periodontol. 2000. 2002; 28: 56–71.
7.
Quirynen M, Teughels W, de Soete M, Steenberghe D. Topical antiseptics and antibiotics in the initial therapy of chronic adult periodontitis: microbiological aspects. Periodontol. 2000. 2002; 28: 72–90.
8.
Adriaens PA, Adriaens LM. Effects of nonsurgical periodontal therapy on hard and soft tissues. Periodontol. 2000. 2004; 36: 121–145.
9.
Umeda M, Takeuchi Y, Noguchi K, Huang Y, Koshy G, Ishikawa I. Effects of nonsurgical periodontal therapy on the microbiota. Periodontol. 2000. 2004; 36: 98–120.
10.
Forabosco A. A comparative study between different techniques in non-surgical periodontal treatment. Minerva Stomatol. 2006; 55: 289–296.
11.
Wyganowska-Świątkowska M, Jaskuła J, Więckowska B. Professional subgingival irrigation in periodontal treatment. Polish J Eviront Stud. 2008; 17: 85–89.
12.
Bosshardt DD. Biological mediators and periodontal regeneration; a review of enamel matrix proteins at the cellular and molecular levels. J Clin Periodontol. 2008; 35: 87–105.
13.
Gestrelius S, Andersson C, Johansson AC, Persson E, Brodin A, Rydhag L, Hammarstrom L. In vitro studies on periodontal ligament cells and enamel matrix derivative. J Clin Periodontol. 1997; 24: 685–668.
14.
Fincham A, Moradian-Oldak J, Simmer JP, Sarte P, Lau EC, Diekwisch T, Slavkin HC. Self-Assembly of a recombinant amelogenin proteins generates supramolecular structures. J Struct Biol. 1994; 112: 103–109.
15.
Lang NP, Tan WC, Krahenmann MA, Zwahlen M. A systematic review of the effects of full-mouth debridement with and without antiseptics in patients with chronic periodontitis. J Clin Periodontol. 2008; 35(8): 8–21.
16.
Claffey N, Polyzois I, Ziaka P. An overview of nonsurgical and surgical therapy. Periodontol. 2000. 2004; 36: 35–44.
17.
Gutierrez MA, Mellonig JT, Cochran DL. Evaluation of enamel matrix derivative as an adjunct to non-surgical periodontal therapy. J Clin Periodontol. 2003; 30: 739–745.
18.
Ramberg P, Sekino S, Uzel NG, Socransky S, Lindhe J. Bacterial colonization during de novo plaque formation. J Clin Periodontol. 2003; 30: 990–995.
19.
Li J, Helmerhorst EJ, Leone CW, Troxler RF, Yaskell T, Haffajee AD, Socransky SS, Oppenheim FG. Identification of early microbial colonizers in human dental biofilm. J Applied Microbiol. 2004; 97: 1311–1318.
20.
Sculean A, Auschill TM, Brecx M, Aeweiler NB. Effect of an enamel matrix protein derivative (Emdogain) on ex vivo dental plaque vitality. J Clin Periodontol. 2001; 28: 1074–1078.
21.
Spahr A, Lyngstadaas SP, Boeckh C, Andersson C, Podbielski A, Haller B. Effect of the enamel matrix derivative Emdogain on the growth of periodontal pathogens in vitro. J Clin Periodontol. 2002; 29: 62–72.
22.
Arweiler NB, Auschill TM, Donos N, Sculean A. Antibacterial effect of an enamel matrix protein derivative on in vitro dental plaque biofilm vitality. Clin Oral Invest. 2002; 6: 205–209.
23.
Walter C, Jawor P, Bernimoulin JP, Hagewald S. Moderate effect of enamel matrix derivative (Emdogain Gel) on Porphyromonas gingivalis growth in vitro. Arch Oral Biol. 2006; 51: 171–176.
24.
Newman SA, Coscia SA, Jotwani R, Iacono VJ, Cutler CW. Effects of Enamel Matrix Derivative on Porphyromonas gingivalis. J Periodontol. 2003; 74(8): 1191–1195.
25.
Laaksonen M, Salo T, Vardar-Sengul S, Atilla G, Han Saygan B, Simmer JP, Baylas H, Sorsa T. Gingival crevicular fluid can degrade Emdogain and inhibit Emdogain-induced proliferation of ligament fibroblasts. J Periodontol Res. 2010; 45: 353–360.
26.
Inaba H, Kawai S, Nakayama K, Okahashi N, Amano A. Effect of Enamel Matrix Derivative on periodontal ligament cells in vitro is diminished by Porphyromonas gingivalis. J Periodontol. 2004; 75(6): 858–865.
27.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent Jr RL. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998; 25: 134–144.
28.
Sanz M, van Winkelhoff AJ. Periodontal infections: understanding the complexity – Consensus of the Seventh European Workshop on Periodontology. J Clin Periodontol. 2011; 38: 3–6.
29.
Wade WG. Has the use of molecular methods for the characterization of the human oral macrobiome changed our understanding of the role of bacteria in pathogenesis of periodontal disease? J Clin Peiodontol. 2011; 38: 7–16.
30.
Andrian E, Grenier D, Rouabhia M. In vitro models of tissue penetration and destruction by Porphyromonas gingivalis. Infect Immunol. 2004; 72(8): 4689–4698.
31.
Valm AM, Welch JL, Rieken CW, Hasegawa Y, Sogin ML, Oldenbourg R, Dewhrist FE, Borisy GG. Systems-level analysis of microbial community organization through combinatorial labeling and spectral imaging. PNAS. 2011; 108: 4152–4157.
32.
Fux CA, Costeron JW, Stewart PS, Stoodley P. Survival strategies of infectious biofilms. Trends Microbiol. 2005; 13(1): 34- 40.
33.
Behl Y, Siqueira M, Ortiz J, Li J, Desta T, Faibish D, Graves DT. Activation of the acquired immune response reduces coupled bone formation in response to a periodontal pathogen. J Immunol. 2008. 181: 8711–8718.
34.
Johnson JD, Chen R, Lenton PA, Zhang G, Hinrichs JE, Rudney JD. Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis. J Periodontol. 2008; 79: 2305–2312.
35.
Sbordonte L, Ramaglia L, Gulletta E, Iacono V. Recolonization of the subgingival microflora after scaling and root planing in human periodontitis. J Periodontol. 1990; 61: 579–584.
| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
