This study tested the effectiveness of benzyl benzoate (BB) in killingTyrophagus putrescentiae (Schrank) mites when using the method utilized by regular consumers. The efficacyof the BB sprays was determined in laboratory experiments and semi-field experiments with mattress andmattress pad pieces. The mites were counted and their living status determined at different time pointsmicroscopically. In the laboratory experiment, the sprays containing either 0.5 %/0.9 % BB with 70 %ethanol or 0.1 % BB with absolute ethanol were highly efficient, resulting in over 90 % mite mortalitywithin 20-30 minutes. In the semi-field experiment, mimicking the home application, the sprays were appliedto pieces of a mattress and a mattress pad, and allowed to affect the area for 30 minutes before thoroughvacuuming. The recovery of mites was usually less than 10 %. The sprays containing BB were effectivein killing the mites in the laboratory, but success was less prominent in the semi-field tests. Thismethod could be used in testing other compounds for their efficacy in killing mites.