Online first
Current issue
Archive
Special Issues
About the Journal
Publication Ethics
Anti-Plagiarism system
Instructions for Authors
Instructions for Reviewers
Editorial Board
Editorial Office
Contact
Reviewers
All Reviewers
2023
2022
2021
2020
2019
2018
2017
2016
General Data Protection Regulation (RODO)
RESEARCH PAPER
Serological and nested PCR survey to determine the occurrence of chlamydia infections in the Polish cattle population
1
Laboratory of Serological Diagnosis /Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Puławy, Poland
2
Department of Allergy and Environmental Health, Institute of Rural Health, Lublin, Poland
Corresponding author
Monika Szymańska-Czerwińska
Laboratory of Serological Diagnosis /Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Puławy, Poland
Laboratory of Serological Diagnosis /Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Puławy, Poland
Ann Agric Environ Med. 2013;20(4):682-686
KEYWORDS
ABSTRACT
Chlamydia spp. is an obligate intracellular agent that causes chlamydiosis in animals and humans. The aim of the presented study was to investigate the prevalence of Chlamydia infection in the Polish cattle population, both asymptomatic and having reproductive disorders. The study was performed on 4,475 serum samples collected from 16 Polish provinces at the turn of 2009–2011. The samples (3,419 from asymptomatic cattle and 1,056 from cattle with reproductive disorders) were tested by complement fixation test (CFT). Moreover, 160 and 201 samples of biological materials from both groups of cattle, respectively, were tested by nested PCR. The results obtained for two tested groups were compared by χ2 (ch-squared) test, both individually for each region (province), and generally for the whole country. The CFT results showed that the seroprevalence of Chlamydia spp. infections in the asymptomatic cattle population was 4.15%, while in the cattle with reproductive disorders – 7.20%. There was a significant statistical difference between compared groups for whole country, but there were no significant differences for individual provinces. The results of PCR showed that Chlamydia spp. was present in both asymptomatic cattle and cattle having reproductive disorders. The nested PCR study confirmed the presence of Chlamydia abortus and Chlamydia suis in the tested samples. The presented study indicates that infections with Chlamydia spp. are present among Polish cattle, but the percentage of infected animals is not high.
REFERENCES (21)
1.
wheelhouse N, Longbottom D. Endemic and emerging chlamydial infections of animals and their zoonotic implications. Transbound Emerg Dis. 2012; 59: 283–291.
2.
Markey B. Chlamydial infections in cattle: The burden of proof? Vet J. 2011; 3: 246–247.
3.
Jee JJ, Degraves FJ, Kim TE, Kaltenboeck B. High prevalence of Natural Chlamydophila species infection in calves. JCM. 2004; 42, 5664–5672.
4.
Pantchev A, Sting R, Bauerfeind R, Tyczka J, Sachse K. Detection of all Chlamydophila and Chlamydia spp. of veterinary interest using species-specific real-time PCR assays. Comp Immunol Microbiol Infect Dis. 2010; 33: 473–484.
5.
Reinhold P, Sachse K, Kaltenboeck B. Chlamydiaceae in cattle: commensals, tigger organisms, or pathogens?. Vet J. 2011; 189: 257–267.
6.
Sachse K, Vretou E, Livingstone M, Borel N, Pospischil A, Longbottom D. Recent developments in the laboratory diagnosis of chlamydial infections. Vet Microbiol. 2009; 135: 2–21.
7.
Livingstone M, Longbottom D. what is the prevalence and economic impact of chlamydial infections in cattle? The need to validate and harmonise existing methods of detection. Vet J. 2006; 172: 3–5.
8.
Pospischil A, Thoma R, Hilbe M, Zimmermann D, Gebbers JO. Abortion in humans caused by Chlamydophila abortus (Chlamydophila psittaci serovar 1) Schweiz Arch Tierheilkd. 2002; 144: 463–466.
9.
welder G, Hotzel H, brezinka C, Gritsch w, Tauber R, würzner R, Ploner F. An unusal cause of sepsis during pregnancy: recognizing infection with Chlamydophila abortus. Obset Gynecol. 2005; 106: 1215–1217.
10.
OIE. Manual of Diagnostic Tests and Vaccines for terrestrial Animals (mammals, birds and bees), Vol. 2, chapter 2.7.7. Enzootic abortion ewes. www.oie.int/fileadmin/home/eng/health_standards/tahm/2.07.07_enz_abor.pdf. (access: May, 2012).
11.
Niemczuk K. Prevalence of antibodies against Chlamydophila psittaci and Chlamydophila abortus in cattle in Poland. A preliminary report. Bull Vet Inst Pulawy. 2005; 49: 293–297.
12.
Niemczuk K. Occurrence of antibodies anti-Chlamydophila sp. in selected cattle’s population in Poland. Med weter. 2006; 61: 804–806.
13.
Borel N, Thoma R, Spaeni P, weilenmann R, Teankum K, Brugnera E, Zimmermann DR, Pospischil A. Chlamydia-related abortions in cattle from Graubunden, Switzerland. Vet Pathol. 2006; 43: 702–708.
14.
Kauffold J, Henning K, Bachmann R, Hotzel H, Melzer F. The prevalence of chlamydiae of bulls from six bull studs in Germany. Anim Reprod Sci. 2007; 102: 111–121.
15.
wang FI, Shieh H, Liao YK. Prevalence of Chlamydophila abortus infection in domesticated ruminants in Taiwan. J Vet Med Sci. 2001; 63: 1215–1220.
16.
wilson K, Sammin D, Harmeyer S, Nath M, Livingstone M, Longbottom D. Seroprevalence of chlamydial infection in cattle in Ireland. Vet J. 2012; 193: 583–585.
17.
Cavirani S, Cabassi CS, Donofrio G, De Iaco B, Taddei S, Flammini CF. Association between Chlamydia psittaci seropositivity and abortion in Italian dairy cows. Prev Vet Med. 2001; 50: 145–151.
18.
Niemczuk K, Truszczyński M. Pathogenicity of Chlamydiaceae with reference to cattle. Med weter. 2012; 68: 328–332.
19.
Kaltenboeck B. Hehnen HR, Vaglenov A. Bovine Chlamydophila spp. infection: do we underestimate the impact on fertility? Vet Res Commun. 2005; 29, 1–15.
20.
Reinhold P, Jaeger J, Liebler-Tenorio E, Berndt A, Bachmann R, Schubert E, Melzer, F, Elschner M, Sachse K. Impact of latent infections with Chlamydophila species in young cattle. Vet J. 2008; 175: 202–211.
21.
Godin ACH, Björkman C, Englund S, Johansson KE, Niskanen R, Alenius S. Investigation of Chlamydophila spp. in dairy cows with reproductive disorders. AVS. 2008; 50:39.
Share
RELATED ARTICLE
| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
