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BRIEF COMMUNICATION
Prevalence of Listeria monocytogenes in sprouts and vegetable mixtures and salads sold in retail shops in Lublin, eastern Poland – preliminary study
1
Department of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, Poland
Corresponding author
Teresa Kłapeć
Department of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, Poland, Jaczewskiego 2, 20-090, Lublin, Poland
Department of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, Poland, Jaczewskiego 2, 20-090, Lublin, Poland
KEYWORDS
TOPICS
ABSTRACT
Introduction:
In 2022–2023, examinations were carried out for the presence of a pathogenic bacterium Listeria monocytogenes in ready-to-eat (RTE) vegetable products (sprouts and vegetable mixtures and salads) sold for immediate consumption in retail shops located in Lublin, eastern Poland. The identification of Listeria strains were performed according to the Polish Standard and accomplished with the Microgen Listeria-ID System.
Results:
A high prevalence of L. monocytogenes infections was found in the unprocessed sprouts of plants belonging to the cabbage (Brassicaceae) family – kale (30.8%), broccoli (18.8%) and radish (7.1%). The mean prevalence of infections in this family (18.6%) was significantly higher compared to the sprouts of plants belonging to other families, none of which were infected (P<0.05). In the minimally processed RTE mixtures and salads, L. monocytogenes was detected in 6.1% of samples. The presence of following non-pathogenic species of the Listeria genus was also found In the examined RTE products: L. innocua, L. grayi, and L. seeligeri, which occurred both in sprouts and vegetable mixtures and salads in the prevalence of 13.8% and 10.2%, respectively.
Conclusions:
The obtained results demonstrate a high prevalence of L. monocytogenes in sprouts, especially those of the cabbage family, which was markedly greater compared to vegetable mixtures. The results suggest a need for continuation of research on the occurrence of L. monocytogenes in the sprouts of plants belonging to the cabbage family. If the results of this study are subsequently confirmed, the application of suitable prevention measures are to be highly recommended.
In 2022–2023, examinations were carried out for the presence of a pathogenic bacterium Listeria monocytogenes in ready-to-eat (RTE) vegetable products (sprouts and vegetable mixtures and salads) sold for immediate consumption in retail shops located in Lublin, eastern Poland. The identification of Listeria strains were performed according to the Polish Standard and accomplished with the Microgen Listeria-ID System.
Results:
A high prevalence of L. monocytogenes infections was found in the unprocessed sprouts of plants belonging to the cabbage (Brassicaceae) family – kale (30.8%), broccoli (18.8%) and radish (7.1%). The mean prevalence of infections in this family (18.6%) was significantly higher compared to the sprouts of plants belonging to other families, none of which were infected (P<0.05). In the minimally processed RTE mixtures and salads, L. monocytogenes was detected in 6.1% of samples. The presence of following non-pathogenic species of the Listeria genus was also found In the examined RTE products: L. innocua, L. grayi, and L. seeligeri, which occurred both in sprouts and vegetable mixtures and salads in the prevalence of 13.8% and 10.2%, respectively.
Conclusions:
The obtained results demonstrate a high prevalence of L. monocytogenes in sprouts, especially those of the cabbage family, which was markedly greater compared to vegetable mixtures. The results suggest a need for continuation of research on the occurrence of L. monocytogenes in the sprouts of plants belonging to the cabbage family. If the results of this study are subsequently confirmed, the application of suitable prevention measures are to be highly recommended.
REFERENCES (29)
1.
Townsend A, Strawn LK, Chapman BJ, et al. A systematic review of Listeria species and Listeria monocytogenes prevalence, persistence, and diversity throughout the fresh produce supply chain. Foods. 2021;10:1427. https://doi.org/10.3390/foods1....
2.
Orsi RH, Liao J, Carlin CR, Wiedmann M. Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022. mBio 2024;15(2):1–15. 10.1128/mbio.00938–23 1.
3.
Buchanan RL, Gorris LGM, Hayman MM, et al. A review of Listeria monocytogenes: An update on outbreaks, virulence, dose-response, ecology, and risk assessments. Food Control. 2017;75:1–13. http://dx.doi.org/10.1016/j.fo....
4.
Chen M, Chen Y, Wu Q, et al. Genetic characteristics and virulence of Listeria monocytogenes isolated from fresh vegetables in China. BMC Microbiol. 2019;19:119. https://doi.org/10.1186/s12866....
6.
Narodowy Instytut Zdrowia Publicznego PZH – Państwowy Instytut Badawczy. Choroby Zakaźne i Zatrucia w Polsce w 2022 r. Warszawa 2023 (in Polish).
7.
Truong HN, Garmyn D, Gal L, et al. Plants as a realized niche for Listeria monocytogenes. MicrobiologyOpen 2021;10(6):e 1255. https://doi.org/10.1002/mbo3.1....
8.
FDA. U. S. Food and Drug Administration 2017. FY 2014–2016 Microbiological Sampling Assignment. Summary Report: Sprouts. Office of Compliance Center for Food Safety and Applied Nutrition.
9.
Thunberg RL, Tran TT, Bennett RW. Microbial evaluation of selected fresh produce obtained at retail markets. J Food Prot. 2002; 65(4):677–682. doi:10.4315/0362-028x-65.4.677.
10.
Kordowska-Wiater M, Janas P, Sosnowska B, et al. Occurrence of pathogenic bacteria and faecal indicators in frozen vegetables. Żywność. Nauka. Technologia. Jakość. 2007;2(51):134–144 (in Polish).
11.
Ponniah J, Robin T, Paie M S, et al. Listeria monocytogenes in raw salad vegetables sold at retail level in Malaysia. Food Control. 2010;21:774–778. https://doi:10.1016/j.foodcont....
12.
De Oliveira MA, De Souza VM, Bergamini AM, et al. Microbiological quality of ready-to-eat minimally processed vegetables consumed in Brazil. Food Control. 2011;22(8):1400–1403. https://doi:10.1016/j.foodcont....
13.
Gurler Z, Pamuk S, Yildirim Y, Ertas, N. The microbiological quality of ready-to-eat salads in Turkey: A focus on Salmonella spp. and Listeria monocytogenes. Int J Food Microbiol. 2015;196:79–83. https://doi.org/10.1016/j.ijfo....
14.
Szymczak B, Szymczak M, Trafiałek J. Prevalence of Listeria species and L. monocytogenes in ready-to-eat foods in the West Pomeranian region of Poland: Correlations between the contamination level, serogroups, ingredients, and producers. Food Microbiol. 2020;91:103532. doi:10.1016/j.fm.2020.103532. Epub 2020 Apr 30.
15.
PN-EN ISO 11290-1:2017. Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection method. Edition 2. 2017–07 (in Polish).
16.
NACMCF. Microbiological safety evaluations and recommendations on sprouted seeds. National Advisory Committee on Microbiological Criteria for Foods. Int J Food Microbiol. 1999;52(3):123–153. https://doi: 10.1016/s0168-1605(99)00135-x.
17.
Strapp CM, Shearer AEH, Joerger RD. Survey of retail alfalfa sprouts and mushrooms for the presence of Escherichia coli O157:H7, Salmonella, and Listeria with BAX, and evaluation of this polymerase chain reaction-based system with experimentally contaminated samples. J Food Prot. 2003;66(2):182–187.
18.
Zhang G, Chen Y, Hu L, et al. Survey of foodborne pathogens, aerobic plate counts, total coliform counts, and Escherichia coli counts in leafy greens, sprouts, and melons marketed in the United States. J Food Prot. 2018;81(3): 400–411. https://doi:10.4315/0362-028X.....
19.
BYJU’S. Asteraceae, Brassicaceae, Poaceae – Angiosperm Families. Characteristics of Brassicaceae.2022. https://byjus.com/biology/aste....
20.
Gorski L, Duhe J M, Flaherty D. The use of flagella and motility for plant colonization and fitness by different strains of the foodborne pathogen Listeria monocytogenes. PLoS One 2009;4(4):e5142. https://doi:10.1371/journal.po....
21.
Gorski L, Palumbo J D, Nguyen K D. Strain-specific differences in the attachment of Listeria monocytogenes to alfalfa sprouts. J Food Prot. 2004;67(11):2488–2495.
22.
Kim SA, Kim OM, Rhee MS. Changes in microbial contamination levels and prevalence of foodborne pathogens in alfalfa (Medicago sativa) and rapeseed (Brassica napus) during sprout production in manufacturing plants. Lett Appl Microbiol. 2013;56(1):30–36. https://doi:10.1111/lam.12009.
23.
Prokopowich D, Blank G. Microbiological evaluation of vegetable sprouts and seeds. J Food Prot. 1991;54(7):560–562. doi:10.4315/0362-028X-54.7.560.
24.
Samadpour M, Barbour MW, Nguyen T, et al. Incidence of enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in retail fresh ground beef, sprouts, and mushrooms. J Food Prot. 2006;69(2):441–443. doi:10.4315/0362-028x-69.2.441.
25.
Saroj SD, Shashidhar R, Dhokane V, et al. Microbiological evaluation of sprouts marketed in Mumbai, India, and its suburbs. J Food Prot. 2006;69(10):2515–2518. doi:10.4315/0362-028x-69.10.2515.
26.
Bergšpica I, Ozola A, Miltiņa E, et al. Occurrence of pathogenic and potentially pathogenic bacteria in microgreens, sprouts, and sprouted seeds on retail market in Riga, Latvia. Foodborne Pathog Dis. 2020;17(7):420–428. https://doi:10.1089/fpd.2019.2....
27.
Sagoo S, Little CL, Ward L, et al. Microbiological study of ready-to-eat salad vegetables from retail establishments uncovers a national outbreak of salmonellosis. J Food Prot. 2003;66(3):403–409. doi:10.4315/0362-028x-66.3.403.
28.
McLauchlin J, Aird H, Amar CFL, et al. Microbiological quality of Ready-to-Eat salad products collected from retail and catering settings in England during 2020 to 2021. J Food Prot. 2022;85(12):1680–1689. https://doi.org/10.4315/JFP-22....
29.
Seo YH, Jang JH, Moon KD. Microbial evaluation of minimally processed vegetables and sprouts produced in Seoul, Korea. Food Sci Biotechnol. 2010;19(5):1283–1288. https:// doi:10.1007/s10068-010-0183-y.
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