Twenty one samples of various materials (organic dust, litter, debris and residues) from farming environments (cowsheds, stables, barns, chaff-cutters, lofts, poultry houses and pigeon houses) were subjected to acarological examination. The samples were collected in Solarnia (near Lubliniec), Kokotek vicinity (province Częstochowa), Łazy (near Książ Wielki province Kielce) and Lesko (province Krosno), all located in southern Poland. A total of 890 mites were isolated including 225 (25.3%) specimens from the order Astigmata (16 species) which comprised species considered as allergenic (e.g. Lepidoglyphus destructor and Glycyphagus domesticus). Species of the family Glycyphagidae (G. domesticus, Glycyphagus privatus, Ctenoglyphus plumiger and L. destructor) have been found as numerically dominant among astigmatic mites.