Introduction and objective:
Elimination irrigation therapy has been proposed as a potential treatment for upper respiratory tract infections, particularly after the COVID-19 pandemic, due to its antiviral properties and mechanical cleansing effects. Additionally, a combination of mineral water with antibiotic therapy has shownto be effective in improving the course clinical infection and positively impact the immune system, potentially enhancing the normal state of microbiota state. The aim of the study is to investigate the influence of elimination-irrigation therapy using a combination of mineral water and ciprofloxacin on the interaction form of individual microbiota species of the upper respiratory tract mucous membrane.

Material and methods:
During the study, microbiological methods were used, such as microscopic, bacteriological and biofilm cultivation methods. rovoking antagonistic interactions within these associations, and a general decrease in microbial colonization activity. During the multispecies biofilm formation by L. sporogenes and S. aureus ATCC 25923, as well as L. sporogenes and E. coli ATCC 25922, a mutual antagonistic effect was determined. The forms of microbial interaction in multispecies biofilm was changed in the presence of 0.0313 mg/ml Ciprofloxacin.

Conclusions:
The 10-minute mineral water treatment and addition of 0.0313 mg/ml Ciprofloxacin enhanced the antagonistic interaction between L. sporogenes and E. coli ATCC 25922, as well as with S. aureus ATCC 25923.