A total of 50 samples of poultry feed mixtures of Slovak origin were analysedfor fumonisin B₁ and B₂ (FB₁, FB₂) and moniliformin (MON) using SAX-clean up procedure beingdetected by high pressure liquid chromatography with mass spectrometry (HPLC-MS) and diode array detection(HPLC-DAD), respectively. The samples were also simultaneously investigated for Fusarium species occurrence,and for the capability of Fusarium isolates recovered to produce FB(1) and MON in vitro. FB1 was detectedin 49 samples (98 %) in concentrations ranging from 43 to 798 microg x kg^-1, and FB₂ in 42 samples(84 %) in concentrations ranging from 26 to 362 microg x kg^-1. MON was detected in 26 samples (52 %)in concentrations that ranged from 42 to 1,214 microg x kg^-1. Only two Fusarium populations were encountered,namely F. proliferatum and F. subglutinans, of which the former was the most dominant and frequent. All86 F. proliferatum isolates tested for FB₁-production ability proved to be producers of the toxin althoughnone of them produced MON. On the contrary, MON production was observed in a half out of 16 F. subglutinans isolates tested, yet no FB₁ production was detected in this case. Despite the limited number of samples investigated during this study, it is obvious that poultry feed mixtures may represent a risk from atoxicological point of view and should be regarded as a potential source of the Fusarium mycotoxins in central Europe. This is the first reported study dealing with fumonisin and moniliformin contamination of poultry feeds from Slovakia.