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RESEARCH PAPER
Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
1
Laboratory of DNA diagnostics, Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
2
Laboratory of Animal Embryology, Institute of Animal Physiology and Genetics, The Academy of Sciences of The Czech Republic, Brno, Czech Republic
3
Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic
Corresponding author
Omar Šerý
Laboratory of DNA diagnostics, Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
Laboratory of DNA diagnostics, Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
Ann Agric Environ Med. 2015;22(4):637-641
KEYWORDS
ABSTRACT
Introduction:
During 2008–2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ).
Objective:
The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing.
Material and Methods:
DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database.
Results:
A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also observed: B. afzelii (70.0%), B. garinii (10.0%), B. valaisiana (8.6%), and B. burgdorferi s. s. (4.3%).
Conclusions:
A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of B. spielmanii for the first time in this region.
During 2008–2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ).
Objective:
The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing.
Material and Methods:
DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database.
Results:
A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also observed: B. afzelii (70.0%), B. garinii (10.0%), B. valaisiana (8.6%), and B. burgdorferi s. s. (4.3%).
Conclusions:
A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of B. spielmanii for the first time in this region.
REFERENCES (40)
1.
Cisak E, Wójcik-Fatla A, Stojek N, Chmielewska-Badora J, Zwoliński J, Buczek A, et al. Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (eastern Poland). Ann Agric Environ Med. 2006; 13(2): 301–306.
2.
Cisak E, Chmielewska-Badora J, Zwoliński J, Wójcik-Fatla A, Polak J, Dutkiewicz J. Risk of tick-borne bacterial diseases among workers of Roztocze National Park (south-eastern Poland). Ann Agric Environ Med. 2005; 12(1): 127–132.
3.
Čapková L, Žakovská A, Knoz J, Dendis M, Šerý O. 2002: Further findings of spirochaetal microorganisms in mosquitoes and ticks in the Czech Republic. Biologia Bratislavae. 2002; 57(3): 389–394.
4.
Žakovska A, Jorková M, Šery O, Dendis M. Spirochaetes in Culex (C.) pipiens s.l. larvae Biologia. 2004; 59(2): 283–287.
5.
Žákovská A, Čapková L, Šerý O, Halouzka J, Dendis M. Isolation of Borrelia afzelii from overwintering Culex pipiens biotype molestus mosquitoes. Ann Agric Environ Med. 2006; 13(2): 345–348.
6.
Netušil J, Žákovská A, Vostal K, Norek A, Stanko M. The occurrence of Borrelia burgdorferi sensu lato in certain ectoparasites (Mesostigmata, Siphonaptera) of Apodemus flavicollis and Myodes glareolus in chosen localities in the Czech Republic. Acta Parasitol. 2013; 58(3): 337–341.
7.
Nejedlá P, Norek A, Vostal K, Žákovská A. What is the percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae? Ann Agric Environ Med. 2009; 16: 273–276.
8.
Richter D, Matuschka FR, Spielman A, Mahadevan L. How ticks get under your skin: insertion mechanics of the feeding apparatus of Ixodes ricinus ticks. Proc Biol Sci. 2013; 280(1773): 20131758.
9.
Parola P, Raoult D. Tick-borne bacterial diseases emerging in Europe. Clin Microbiol Infect. 2001; 7(2): 80–83.
10.
Hynote ED, Mervine PC, Stricker RB. Clinical evidence for rapid transmission of Lyme disease following a tickbite. Diagn Microbiol Infect Dis. 2012; 72(2): 188–192.
11.
Crippa M. Rais O, Gern L. Investigations on the mode and dynamics of transmission and infectivity of Borrelia burgdorferi sensu stricto and Borrelia afzelii in Ixodes ricinus ticks. Vector Borne Zoonotic Dis. 2002; 2(1): 3–9.
12.
Rauter C, Hartung T. Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis. Appl Environ Microbiol. 2005; 71(11): 7203–7216.
13.
Rudenko N, Golovchenko M, Grubhoffer L, Oliver JH Jr. Updates on Borrelia burgdorferi sensu lato complex with respect to public health. Ticks Tick Borne Dis. 2011; 2(3): 123–128.
14.
Hubálek, Z, Halouzka J, Juřicová Z. Longitudinal surveillance of the ticks Ixodes ricinus for borreliae. Med Vet Entomol. 2003; 17:46–51.
16.
Smetanová K, Burri C, Pérez D, Gern L, Kocianová E. Detection and identification of Borrelia burgdorferi sensu lato genospecies in ticks from three different regions in Slovakia. Wien Klin Wochenschr. 2007; 119(17–18): 534–537.
17.
Hubálek Z, Halouzka J, Juřicová Z. Borreliae in Ixodes ricinus ticks feeding on humans. Med Vet Entomol. 2004; 18(3): 228–231.
18.
Franke J, Hildebrandt A, Meier F, Straube E, Dorn W. Prevalence of Lyme disease agents and several emerging pathogens in questing ticks from the German Baltic coast. J Med Entomol. 2011; 48(2): 441–444.
19.
Institute of Health Information and Statistics of the Czech Republic: Infekční nemoci 2012 http://www.uzis.cz/system/file... (access: 2014.06.20) (in Czech).
20.
The National Institute of Public Health http://www.szu.cz/publikace/da... (access: 2014.06.20) (in Czech).
21.
Žákovská A, Netušil J, Martiníková H. Statistical evaluation of the occurrence of Ixodes ricinus ticks in the urban locality of Brno – Pisárky and the influence of some environment factors on their presence. J Vector Ecol. 2007; 32(1): 29–33.
22.
Žákovská A, Nejezchlebová H, Bartoňková N, Rašovská T, Kučerová H, Norek A, et al. Activity of the tick Ixodes ricinus monitored in a suburban park in Brno, Czech Republic, in association with evaluation of selected repellents. J Vector Ecol. 2013; 38(2): 295–300.
23.
Cisak E, Wójcik-Fatla A, Zając V, Dutkiewicz J. Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases. Ann Agric Environ Med. 2012;19(4): 625–630.
24.
Rizzoli A, Hauffe H, Carpi G, Vourc HG, Neteler M, Rosa R. Lyme borreliosis in Europe. Euro Surveill. 2011; 16(27): pii:19906.
25.
Žákovská A, Janouškovcová E, Pejchalová K, Halouzka J, Dendis M. Identification and characterization of 31 isolates of Borrelia burgdorferi (Spirochaetales, Spirochaetaceae) obtained from various hosts and vectors using PCR-RFLP and SDS-PAGE analysis. Acta Parasitologica. 2008; 53(2): 186–192.
26.
Bašta J, Plch J, Hulínská D, Daniel M. Incidence of Borrelia garinii and Borrelia afzelii in Ixodes ricinus ticks in an urban environment, Prague, Czech Republic, between 1995 and 1998. Eur J Clin Microbiol Infect Dis. 1999; 18(7): 515–517.
27.
Niścigorska-Olsen J, Wodecka B, Moranska I, Skotarczak B. Genospecies of Borrelia burgdorferi sensu lato in patients with erythema migrans. Ann Agric Environ Med. 2008; 15(1): 167–170.
28.
Derdáková M, Beati L, Pet‘ko B, Stanko M, Fish D. Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in ixodes ricinus ticks from the Czech Republic. Appl Environ Microbiol. 2003; 69(1): 509–516.
29.
Richter D, Schlee DB, Allgöwer R, Matuschka FR. Relationships of a novel Lyme disease spirochete, Borrelia spielmani sp. nov., with its hosts in Central Europe. Appl Environ Microbiol. 2004; 70(11): 6414–6419.
30.
Richter D, Postic D, Sertour N, Livey I, Matuschka FR, Baranton G. Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov. Int J Syst Evol Microbiol. 2006; 56(Pt 4): 873–881.
31.
Földvári G, Farkas R, Lakos A. Borrelia spielmanii erythema migrans, Hungary. Emerg Infect Dis. 2005; 11(11): 1794–1795.
32.
Maraspin V, Ruzic-Sabljic E, Strle F. Lyme borreliosis and Borrelia spielmanii. Emerg Infect Dis. 2006; 12(7): 1177.
33.
Pejchalová K, Žákovská A, Mejzlíková M, Halouzka J, Dendis M. Isolation, cultivation and identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from the city of Brno, Czech Republic. Ann Agric Environ Med. 2007; 14(1): 75–79.
34.
Schutzer SE, Fraser-Liggett CM, Qiu WG, Kraiczy P, Mongodin EF, Dunn JJ, et al. Whole-genome sequences of Borrelia bissettii, Borrelia valaisiana, and Borrelia spielmanii. J Bacteriol. 2012; 194(2): 545–546.
35.
Stĕpánová-Tresová G, Kopecký J, Kuthejlová M. Identification of Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii in Ixodes ricinus ticks from southern Bohemia using monoclonal antibodies. Zentralbl Bakteriol. 2000; 289(8): 797–806.
36.
Danielová V, Daniel M, Rudenko N, Golovchenko M. Prevalence of Borrelia burgdorferi sensu lato genospecies in host-seeking Ixodes ricinus ticks in selected South Bohemian locations (Czech Republic). Cent Eur J Public Health. 2004; 12(3): 151–156.
37.
Pangrácová L, Derdáková M, Pekárik L, Hviščová I, Víchová B, Stanko M, et al. Ixodes ricinus abundance and its infection with the tick-borne pathogens in urban and suburban areas of Eastern Slovakia. Parasit Vectors. 2013; 6(1): 238.
38.
Cisak E, Wójcik-Fatla A, Stojek N, Chmielewska-Badora J, Zwoliński J, Buczek A, et al. Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (eastern Poland). Ann Agric Environ Med. 2006; 13(2): 301–30.
39.
Stünzner D, Hubálek Z, Halouzka J, Postic D, Pierer K, Marth E. Prevalence of Borrelia burgdorferi s.I. in Ixodes ricinus ticks from Styria (Austria) and species identification by PCR-RFLP analysis. Zentralbl Bakteriol. 1998; 288(4): 471–478.
40.
Hildebrandt A, Schmidt KH, Wilske B, Dorn W, Straube E, Fingerle V. Prevalence of four species of Borrelia burgdorferi sensu lato and coinfection with Anaplasma phagocytophila in Ixodes ricinus ticks in central Germany. Eur J Clin Microbiol Infect Dis. 2003; 22(6): 364–367.
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