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BRIEF COMMUNICATION
Host response to Borrelia afzelii in BALB/c mice tested by immunoblotting
1
Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
Ann Agric Environ Med. 2013;20(4):823-825
KEYWORDS
ABSTRACT
The aim was to find if there is variability in the production of specific antibodies by BALB/c mice to certain antigens of three individual Borrelia afzelii (dead cell suspension) strains originally isolated from different sources. Analysis of the borrelian proteins immunogenicity was performed and determined to particular strains by immunoblotting. Some differences in production of specific IgM and IgG antibodies in each individual group of tested mice were found. The antigenic influence of OspA and OspC proteins appeared to be the most important for the induction of protective immune reaction. OspC was produced both in early and late infection of all individuals and OspA induced production of IgG antibodies in all groups of immunized mice.
REFERENCES (20)
1.
Mannelli A, Bertolotti L, Gern L, Gray J. Ecology of Borrelia burgdorferi sensu lato in Europe: transmission dynamics in multi-host systems, influence of molecular processes and effects of climate change. FEMS Microbiol Rev. 2012; 36(4): 837–861.
2.
Kempf F, De Meeûs T, Vaumourin E, Noel V, Taragel’ová V, Plantard O, Heylen DJ, Eraud C, Chevillon C, McCoy KD. Host races in Ixodes ricinus, the European vector of Lyme borreliosis. Infect Genet Evol. 2011; 11(8): 2043–2048.
3.
Poljak A, Comstedt P, Hanner M, Schüler W, Meinke A, Wizel B, Lundberg U. Identification and characterization of Borrelia antigens as potential vaccine candidates against Lyme borreliosis. Vaccine. 2012; 30(29): 4398–406.
4.
Wilske B, Hauser U, Lert G, Jauris-Heipke S. Genospecies and their influence on immunoblot results: Wein klin Wochenschr. 1998; 110(24): 882–885.
5.
Merljak Skocir L, Ruzić-Sabljić E, Maraspin-Carman V, Lotric-Furlan S, Logar M, Strle F. Comparison of different Borrelia burgdorferi sensu lato strains for detection of immune response in patients with erythema migrans. Int J Med Microbiol. 2008; 298(5–6): 493–504.
6.
Jiang Y, Hou XX, Geng Z, Hao Q, Wan KL. Interpretation criteria for standardized Western blot for the predominant species of Borrelia burgdorferi sensu lato in China. Biomed Environ Sci. 2010; 23(5): 341–349.
7.
Žákovská A, Janouškovcová E, Pejchalová K, Halouzka J. Identification and characterization of 31 isolates of Borrelia burgdorferi (Spirochaetales, Spirochaetaceae) obtained from various hosts and vectors using PCR-RFLP and SDS-PAGE analysis. Acta Parasitologica. 2008; 53(2): 186–192.
8.
Žákovská A, Rusňáková H, Nejedlá P, Vostal K. Borrelia afzelii and immune response of BALB/c mice. Acta Vet Brno. 2011; 80(4): 325–329.
9.
Battisti JM, Bono JL, Rosa PA, Schrumpf ME, Schwan TG, Policastro PF. Outer surface protein A protects Lyme disease spirochetes from acquired host immunity in the tick vector. Infect Immun. 2008; 76(11): 5228–5237.
10.
Neelakanta G, Li X, Pal U, Liu X, Beck DS, DePonte K, Fish D, Kantor FS, Fikrig E. Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks. PLoS Pathog. 2007; 3(3): e33.
11.
Schwan TG, Burgdorfer W. Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis. 1987; 156(5): 852–853.
12.
Seemanapalli SV, Xu Q, McShan K, Liang FT. Outer surface protein C is a dissemination-facilitating factor of Borrelia burgdorferi during mammalian infection. PLoS One. 2010; 5(12): e15830.
13.
Lovrich SD, Jobe DA, Schell RF, Callister SM. Borreliacidal OspC antibodies specific for a highly conserved epitope are immunodominant in human Lyme disease and do to occur in mice or hamsters. Clin Diagn Lab Immunol. 2005; 12(6): 746–751.
14.
Li X, Neelakanta G, Liu X, Beck DS, Kantor FS, Fish D, Anderson JF, Fikrig E. Role of outer surface protein D in the Borrelia burgdorferi life cycle. Infect Immun. 2007; 75(9): 4237–4244.
15.
Hellwage J, Meri T, Heikkila T, Alitalo A, Panelius J, Lahdenne P, Seppala IJ, Meri S. The complement regulator factor H binds to the surface protein OspE Borrelia burgdorferi. J Biol Chem. 2001; 276(11): 8427–8435.
16.
Hulínská D. Laboratorní dignostika. In: Bartůněk P. Lymeská borrelióza. Grada Publishing, Prague 2001. p. 39–45.
17.
Gilmore RD Jr, Murphree RL, James AM, Sullivan SA, Johnson BJ. The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early Lyme disease in the flaA gene product. J Clin Microbiol. 1999; 37(3): 548–552.
18.
Bryksin AV, Godfrey HP, Carbonaro CA, Wormser GP, AgueroRosenfeld ME, Cabello FC. Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme disease. Clin Diagn Lab Immunol. 2005; 12(8): 935–940.
19.
Humair PF, Rais O, Gern L. Transmission of Borrelia afzelii from Apodemus mice and Clethrionomys voles to Ixodes ricinus ticks: differential transmission pattern and overwintering maintenance. Parasitology. 1999; 118(1): 33–42.
20.
Hulínská D. Diagnosis of Lyme borreliosis with western blotting. Epidemiol Mikrobiol Imunol. 1997; 46(1):3–8. Article in Czech.
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