Online first
Current issue
Archive
Special Issues
About the Journal
Publication Ethics
Anti-Plagiarism system
Instructions for Authors
Instructions for Reviewers
Editorial Board
Editorial Office
Contact
Reviewers
All Reviewers
2023
2022
2021
2020
2019
2018
2017
2016
General Data Protection Regulation (RODO)
RESEARCH PAPER
First molecular characterization of Dobrava-Belgrade virus found in
Apodemus flavicollis in Poland
1
Biological Threats Identification and Countermeasure Centre, Military Institute of Hygiene and Epidemiology, Pulawy, Poland
2
Provincial Sanitary Epidemiological Station, Rzeszow, Poland
3
Department of Zoology, Faculty of Biology and Agriculture, University of Rzeszow, Poland
Corresponding author
Marcin Kolodziej
Biological Threats Identification and Countermeasure Center, Military Institute of Hygiene and Epidemiology, Biological Threats Identification and Countermeasure Center, Military Institute of Hygiene and Epide, 2 Lubelska St., 24-100 Pulawy, Poland
Biological Threats Identification and Countermeasure Center, Military Institute of Hygiene and Epidemiology, Biological Threats Identification and Countermeasure Center, Military Institute of Hygiene and Epide, 2 Lubelska St., 24-100 Pulawy, Poland
Ann Agric Environ Med. 2018;25(2):368-373
KEYWORDS
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industryState of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.
ABSTRACT
Introduction:
Dobrava-Belgrade virus (DOBV) is one of the emerging pathogens which have been reported during the last decades in Europe and have attracted the attention of researchers. The course of infection among humans may have a varied course – from the completely asymptomatic to the more severe forms, such as haemorrhagic fever with renal syndrome (HFRS). DOBV is hosted and carried by rodents like Apodemus flavicollis or A. agrarius, which occur commonly in Europe.
Objective:
To-date, orthohantaviruses have been reported in Poland, both in humans and animals, but detailed country-scale studies have not yet been carried out. The aim of the study was molecular characterization of a strain which was found in A. flavicollis in south-eastern Poland.
Material and methods:
The phylogenetic analysis of the first Dobrava-Belgrade virus found in A. flavicollis in the subcarpathian region of south-eastern Poland, presented in this study, was performed after virus proliferation in cell culture and sequencing of specific PCR products.
Results:
Based on genetic sequences of fragments of three segments (S, M and L), the isolated virus was assigned to the Dobrava genotype, taking into consideration the most current classification of the DOBV species.
Conclusions:
The Dobrava-Belgrade virus strain isolated from A. flavicollis in the subcarpathian region of south-eastern Poland, has been molecularly characterized and assigned to Dobrava genotype, thereby the occurrence of that genotype in Poland has been confirmed by molecular techniques.
Dobrava-Belgrade virus (DOBV) is one of the emerging pathogens which have been reported during the last decades in Europe and have attracted the attention of researchers. The course of infection among humans may have a varied course – from the completely asymptomatic to the more severe forms, such as haemorrhagic fever with renal syndrome (HFRS). DOBV is hosted and carried by rodents like Apodemus flavicollis or A. agrarius, which occur commonly in Europe.
Objective:
To-date, orthohantaviruses have been reported in Poland, both in humans and animals, but detailed country-scale studies have not yet been carried out. The aim of the study was molecular characterization of a strain which was found in A. flavicollis in south-eastern Poland.
Material and methods:
The phylogenetic analysis of the first Dobrava-Belgrade virus found in A. flavicollis in the subcarpathian region of south-eastern Poland, presented in this study, was performed after virus proliferation in cell culture and sequencing of specific PCR products.
Results:
Based on genetic sequences of fragments of three segments (S, M and L), the isolated virus was assigned to the Dobrava genotype, taking into consideration the most current classification of the DOBV species.
Conclusions:
The Dobrava-Belgrade virus strain isolated from A. flavicollis in the subcarpathian region of south-eastern Poland, has been molecularly characterized and assigned to Dobrava genotype, thereby the occurrence of that genotype in Poland has been confirmed by molecular techniques.
REFERENCES (27)
1.
Holmes EC, Zhang Y-Z. The evolution and emergence of hantaviruses. Curr Opin Virol. 2015;10:27-33.
2.
Klempa B, Avšič-Županc T, Clement J, Dzagurowa TK, Henttonen H, Heyman P, et al. Complex evolution and epidemiology of Dobrava-Belgrade hantavirus: definition of genotypes and their characteristics. Arch Virol. 2013;158: 521-529.
3.
Michalski A, Niemcewicz M, Bielawska-Drózd A, Nowakowska A, Gaweł J, Pitucha G, et al. Surveillance of hantaviruses in Poland: a study of animals reservoirs and human hantavirus disease in Subcarpathia. Vector Borne Zoonotic Dis. 2014; 14(7): 514-522.
4.
Klempa B, Schütt M, Auste B, Labuda M, Ulrich R, Meisel H, et al. First molecular identification of human Dobrava virus infection on Central Europe. J Clin Microbiol. 2004; 42(3): 1322-1325.
5.
Avšič-Županc T, Korva M, Markotić A. HFRS and hantaviruses in the Balkans/South-East Europe. Virus Res. 2014; 187: 27-33.
6.
Sadkowska-Todys M, Gut W, Baumann A, Siennicka J, Litwińska B, Zieliński A. Ocena problemu występowania zakażeń ludzi hantawirusami na terenie Polski, ze szczególnym uwzględnieniem wirusa Puumala. [Occurrence of human hantavirus infections in Poland]. Przegl Epidemiol. 2007; 61: 497-503.
7.
Knap JP, Nowakowska A, Dutkiewicz J, Zając V, Wójcik-Fatla A, Chmielewska-Badora J, et al. Obecność przeciwciał anty-hantawirusowych u leśników Roztoczańskiego Parku Narodowego i nadleśnictwa Puławy (makroregion lubelski) Doniesienie wstępne. [Detection of antibodies against hantaviruses in forestry workers of the Roztocze National Park and Puławy Forest Inspectorate (Lublin macroregion)]. Med Ogólna. 2010; 16(2): 201-216.
8.
Song J-W, Baek LJ, Song K-J, Skrok A, Markowski J, Bratosiewicz-Wasik J, et al. Characterization of Tula virus from common voles (Microtus arvalis) in Poland: evidence for geographic-specific phylogenetic clustering. Virus Genes. 2004; 29(2): 239-247.
9.
Wójcik-Fatla A, Zając V, Knap JP, Sroka J, Cisak E, Sawczyn A, et al. A small-scale survey of hantavirus in mammals from eastern Poland. Ann Agric Environ Med. 2013, 20(2): 283-286.
10.
Gu SH, Markowski J, Kang HJ, Hejduk J, Sikorska B, Liberski PP, et al. Boginia virus, a newfound hantavirus harbored by the Eurasian water sire (Neomys fodiens) in Poland. Virol J. 2013; 10(160).
11.
Kramski M, Meisel H, Klempa B, Krüger DH, Pauli G, Nitsche A. Detection and typing of human pathogenic hantaviruses by real-time reverse transcription-PCR and pyrosequencing. Clin Chem. 2007; 53(11): 1899-1905.
12.
Maes P, Keyaerts E, Li S, Nlandu-Masunda V, Clement J, Van Ranst M. Replication reduction neutralization test, a quantitative RT-PCR-based techniques for the detection of neutralizing hantavirus antibodies. J Virol Methods. 2009; 59(2): 295-299.
13.
Papa A, Johnson AM, Stockton PC, Bowen MD, Spiropoulou CF, Alexiou-Daniel S, et al. Retrospective serological and genetic study of the distribution of hantaviruses in Greece. J Med Virol. 1998; 55(4): 321-327.
14.
Klempa B, Stanko M, Labuda M, Ulrich R, Meisel H, Krüger DH. Central European Dobrava hantavirus isolate from a striped field mouse (Apodemus agrarius). J Clin Microbiol. 2005; 43(6): 2756-2763.
15.
Weidmann M, Schmidt P, Vackova M, Krivanec K, Munclinger P, Hufert FT. Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR. J Clin Microbiol. 2005; 43(2): 808-812.
16.
Schlegel M, Klempa B, Schmidt-Chanasit J, Büchner T, Groschup MH, Meier M, et al. Dobrava-Belgrade virus spillover infections, Germany. Emerging Infect Dis. 2009; 15(12): 2017-2020.
17.
Plyusnin A, Krüger DH, Lundkvist A. Hantavirus infections in Europe. Adv Virus Res. 2001; 57: 105-136.
18.
Hofmann J, Meier M, Enders M, Führer A, Ettinger J, Klempa B, et al. Hantavirus disease in Germany due to infection with Dobrava-Belgrade virus genotype Kurkino. Clin Microbiol Infect. 2014; 20: O648-O655.
19.
Jakab F, Horváth G, Ferenczi E, Sebők J, Varecza Z, Szűcs G. Detection of Dobrava hantaviruses in Apodemus agrarius in the Transdanubian region in Hungary. Virus Res. 2007; 128(1-2): 149-152.
20.
Plyusnina A, Krajinović L, Margaletic J, Niemimaa, J., Nemirov, K., Lundkvist Ǻ, et al. Genetic evidence for the presence of two distinct hantaviruses associated with Apodemus mice in Croatia and analysis of local strains. J Med Virol. 2011; 83(1): 108-114.
21.
Avšič-Županc T, Nemirov K, Petroviec M, Trilar T, Poljak M, Vaheri A, et al. Genetic analysis of wild-type Dobrava hantavirus in Slovenia: co-existence of two distinct genetic lineages within the same natural focus. J Gen Virol. 2000; 81(7): 1747-1755.
22.
Klempa B, Schmidt HA, Ulrich R, Kaluz S, Labuda M, Meisel H, et al. Genetic interaction between distinct Dobrava hantavirus subtypes in Apodemus agrarius and A. flavicollis in Nature. J Virol. 2003; 77(1): 804-809.
23.
Sandmann S, Meisel H, Razanskiene A, Wolbert A, Pohl B, Krüger DH, et al. Detection of human hantavirus infection in Lithuania. Infection 2005; 33(2): 66-72.
24.
Kirsanovs S, Klempa B, Franke R, Lee M-H, Schönrich G, Rang A, et al. Genetic reassortment between high-virulent and low-virulent Dobrava-Belgrade virus strains. Virus Genes 2010; 41(3): 319-328.
25.
Krüger DH, Ulrich RG, Hofmann J. Hantaviruses as zoonotic pathogens in Germany. Dtsch Arztebl Int. 2013; 110(27-28): 461-467.
Share
RELATED ARTICLE
| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
