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RESEARCH PAPER
Comparison of the method of quantitative determination of Legionella pneumophila acc.
to PN-EN ISO 11731:2017 with the Legiolert™/Quanti-Tray® (IDEXX)
1
National Institute of Public Health NIH – National Research Institute, Warsaw, Poland
Corresponding author
Izabela Orych
National Institute of Public Health NIH - National Research Institute, 24 Chocimska St., 00-791, Warsaw, Poland
National Institute of Public Health NIH - National Research Institute, 24 Chocimska St., 00-791, Warsaw, Poland
Ann Agric Environ Med. 2024;31(1):24-28
KEYWORDS
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industry
ABSTRACT
Introduction:
Legionella pneumophila is the primary etiological agent of Legionnaires’ disease. These are opportunistic pathogens causing lung infections by inhalation of contaminated aerosols. Controlling the presence of these bacteria in domestic distribution water systems (mainly hot water systems) is important for reducing the threat they pose to human health. Legionella pathogens are detected and quantified during routine testing of water samples according to procedures included in PN-EN ISO 11731:2017. However, these procedures are labour-intensive, and the results are obtained after a relatively long time. Implementing the Legiolert™/Quanti-Tray® test as an alternative method may constitute a good solution: it simplifies the testing procedure and significantly reduces the time necessary to obtain the final result.
Objective:
The aim of the study was to compare the relative recovery of Legionella from water samples tested according to PN-EN ISO 11731:2017, and the alternative method of the most probable number (MPN) with the Legiolert™/Quanti-Tray® (IDEXX) test, and to assess the suitability of the alternative method for routine testing.
Material and methods:
Parallel testing was conducted of 38 hot water samples to detect and determine Legionella acc. to PN-EN ISO 11731:2017 and the Legiolert™/Quanti-Tray® test. Statistical analysis of the results was performed according to PN-EN ISO 17994:2014 and the McNemar’s test.
Results:
The Legiolert™ test was confirmed to be comparable in performance to the reference standardized method in both qualitative and quantitative detection of L. pneumophila in hot water samples.
Conclusions:
The study confirmed that the Legiolert™ test is specific and easy to use, and may constitute an alternative to standardized procedures used in the quantification of L. pneumophila in water.
Legionella pneumophila is the primary etiological agent of Legionnaires’ disease. These are opportunistic pathogens causing lung infections by inhalation of contaminated aerosols. Controlling the presence of these bacteria in domestic distribution water systems (mainly hot water systems) is important for reducing the threat they pose to human health. Legionella pathogens are detected and quantified during routine testing of water samples according to procedures included in PN-EN ISO 11731:2017. However, these procedures are labour-intensive, and the results are obtained after a relatively long time. Implementing the Legiolert™/Quanti-Tray® test as an alternative method may constitute a good solution: it simplifies the testing procedure and significantly reduces the time necessary to obtain the final result.
Objective:
The aim of the study was to compare the relative recovery of Legionella from water samples tested according to PN-EN ISO 11731:2017, and the alternative method of the most probable number (MPN) with the Legiolert™/Quanti-Tray® (IDEXX) test, and to assess the suitability of the alternative method for routine testing.
Material and methods:
Parallel testing was conducted of 38 hot water samples to detect and determine Legionella acc. to PN-EN ISO 11731:2017 and the Legiolert™/Quanti-Tray® test. Statistical analysis of the results was performed according to PN-EN ISO 17994:2014 and the McNemar’s test.
Results:
The Legiolert™ test was confirmed to be comparable in performance to the reference standardized method in both qualitative and quantitative detection of L. pneumophila in hot water samples.
Conclusions:
The study confirmed that the Legiolert™ test is specific and easy to use, and may constitute an alternative to standardized procedures used in the quantification of L. pneumophila in water.
ACKNOWLEDGEMENTS
The authors acknowledge with thanks the funding, supplies,
and technical support received from Dagmara Dziedzina,
Dan Broder, Brett Brewin and Jeff Bates at the IDEXX
Laboratories Inc., (Westbrook, ME, USA).
REFERENCES (38)
1.
Fields BS, Benson RF, Besser RE. Legionella and legionnaires’ disease: 25 years of investigation. Clin Microbiol Rev. 2002;15(3):506–26. https://doi:10.1128/CMR.15.3.5....
2.
McDade JE, Shepard CC, Fraser DW, et al. Isolation of a bacterium and demonstration of its role in other respiratory disease. N Engl J Med. 1977;1;297(22):1197–1203. https://doi.org/10.1056/NEJM19....
3.
European Centre for Disease Prevention and Control. Legionnaires’ disease. In: ECDC. Annual epidemiological report for 2021. Stockholm: https://www.ecdc.europa.eu/sit... (access 2023.08.16).
4.
European Centre for Disease Prevention and Control. Legionnaires’ disease. In: ECDC. Annual epidemiological report for 2020. Stockholm: https://www.ecdc.europa.eu/sit... (access 2023.08.16).
5.
Stallworth C, Steed L, Fisher MA, et al. Legionnaires’ disease caused by Legionella londiniensis. J Clin Microbiol. 2012 Dec;50(12):4178–4179. https://doi.org/10.1128%2FJCM.....
6.
Walker JT, McDermott PJ. Confirming the Presence of Legionella pneumophila in Your Water System: A Review of Current Legionella Testing Methods. J AOAC Int. 2021 Aug 20;104(4):1135–1147. https://doi.org/10.1093/jaoaci....
7.
Chambers ST, Slow S, Thomas AS, et al. Legionellosis Caused by Non-Legionella pneumophila Species, with a Focus on Legionella longbeachae Microorganisms. 2021;9:291. https://doi.org/10.3390/microo....
8.
Ditommaso S, Giacomuzzi M, Memoli G, et al. Comparison of BCYE?+AB agar and MWY agar for detection and enumeration of Legionella spp. in hospital water samples. BMC Microbiol. 2021;21:48. https://doi.org/10.1186/s12866....
9.
Miyashita N, Higa F, Aoki Y, et al. Distribution of Legionella species and serogroups in patients with culture-confirmed Legionella pneumonia. J Infect Chemother. 2020;26:411–417. https://doi.org/10.1016/j.jiac....
10.
National Academies of Sciences, Engineering, and Medicine. 2019. Management of Legionella in Water Systems. Washington, DC: The National Academies Press. http://doi.org/10.17226/25474.
11.
WHO (World Health Organization). 2007. LEGIONELLA and the prevention of legionellosis Edited by: Jamie Bartram, Yves Chartier, John V Lee, Kathy Pond and Susanne Surman-Lee ISBN 92 4 156297 8 (NLM classification: WC 200).
12.
Chauhan DR, Shames SR. Pathogenicity and Virulence of Legionella: Intracellular replication and host response. Virulence. 2021;12(1):1122–1144. https://doi.org/10.1080/215055....
13.
Yu VL, Plouffe JF, Pastoris MC, et al. Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. J Infect Dis. 2002;186(1):127–128. https://doi.org/10.1086/341087.
14.
WHO (World Health Organization). 2017. Drinking Water Parameter Cooperation Project Support to the revision of Annex I Council Directive 98/83/EC on the Quality of Water Intended for Human Consumption (Drinking Water Directive) Recommendations Bonn, 11 September 2017.
15.
Directive (EU) 2020/2184 of the European Parliament and of the Council of 16 December 2020 on the quality of water intended for human consumption.
16.
Scaturro M, Chierico FD, Motro Y, et al. Bacterial communities of premise plumbing systems in four European cities, and their association with culturable Legionella. bioRxiv, 12 Aug 2022. https://doi.org/10.1101/2022.0....
17.
ESGLI (European Study Group for Legionella Infections) 2017. European technical guidelines for the prevention, control and investigation of infections caused by Legionella species. https://www.escmid.org/fileadm... (access 2023.08.16).
18.
Pierre D, Baron JL, Ma X, et al. Water Quality as a Predictor of Legionella Positivity of Building Water Systems. Pathogens. 2019;8:295. https://doi.org/10.3390/pathog....
19.
WHO (World Health Organization). 2011. Water safety in buildings.20. Girolamini L, Salaris S, Pascale MR, et al. Dynamics of Legionella Community Interactions in Response to Temperature and Disinfection Treatment: 7 Years of Investigation. Microb Ecol. 2022;83(2):353–362. https://doi.org/10.1007/s00248....
20.
Sikora A, Wójtowicz-Bobin M, Kozioł-Montewka M, et al. Prevalence of Legionella pneumophila in water distribution systems in hospitals and public buildings of the Lublin region of eastern Poland. Ann Agric Environ Med. 2015;22(2):195–201. https://doi.org/10.5604/123219....
21.
Zimoch I, Paciej J. Analiza przestrzenna ryzyka zagrożenia zdrowotnego wywołanego obecnością bakterii z rodzaju Legionella w instalacjach ciepłej wody w województwie śląskim. Ochrona Środowiska. 2014;36(4):23–28.
22.
Matuszewska R, Szczotko M. Perspektywy długoterminowego zastosowania elektrolitycznej metody dezynfekcji jonami miedzi i srebra do usuwania bakterii Legionella występujących w wodzie w wewnętrznych instalacjach wodociągowych budynków. Gaz, Woda i Technika Sanitarna. 2019;10:320–323. doi.10.15199/17.2019.10.2.
23.
Matuszewska R, Szczotko M. Ocena występowania punktowego i systemowego zanieczyszczenia bakteriami z rodzaju Legionella w instalacjach wodociągowych wody ciepłej. Instal. 2020;11:36–40. doi.10.36119/15.2020.11.5.
24.
PN-ISO 11731:2002 Jakość wody – Wykrywanie i oznaczanie bakterii z rodzaju Legionella. PKN (in Polish).
25.
PN-EN ISO 11731-2:2008 Jakość wody – Wykrywanie i oznaczanie ilościowe bakterii z rodzaju Legionella – Część 2. Metoda filtracji membranowej dla wód o małej liczbie bakterii. PKN (in Polish).
26.
Rozporządzenie Ministra Zdrowia z dnia 29 marca 2007 r. w sprawie jakości wody przeznaczonej do spożycia przez ludzi (DzU 2007 nr 61 poz. 417).
27.
PN-EN ISO 11731:2017-08 Jakość wody – Oznaczanie ilościowe bakterii z rodzaju Legionella. PKN (in Polish).
28.
Rozporządzenie Ministra Zdrowia z dnia 7 grudnia 2017 r. w sprawie jakości wody przeznaczonej do spożycia przez ludzi (DzU 2017 poz. 2294).
29.
PN-EN ISO 19458:2007 Jakość wody – Pobieranie próbek do analiz mikrobiologicznych. PKN (in Polish).
30.
PN-EN ISO 17994:2014 Jakość wody – Wytyczne do porównania względnego odzysku mikroorganizmów według dwóch metod ilościowych. PKN (in Polish).
31.
Boczek LA, Tang M, Formal C, et al. Comparison of two culture methods for the enumeration of Legionella pneumophila from potable water samples. J Water Health. 2021;19(3):468–477. https://doi.org/10.2166/wh.202....
32.
Rech MM, Swalla BM, Dobranic JK. Evaluation of Legiolert for Quantification of Legionella pneumophila from Non-potable Water. Curr Microbiol. 2018;75(10):1282–1289. https://doi.org/10.1007/s00284....
33.
Scaturro M, Buffoni M, Girolamo A, et al. Performance of Legiolert Test vs. ISO 11731 to Confirm Legionella pneumophila Contamination in Potable Water Samples. Pathogens. 2020;9(9):690. https://doi.org/10.3390/pathog....
34.
Spies K, Pleischl S, Lange B, et al. Comparison of the LegiolertTM/Quanti-Tray® MPN test for the enumeration of Legionella pneumophila from potable water samples with the German regulatory requirements methods ISO 11731–2 and ISO 11731. Int J Hyg Environ Health. 2018;221:1047–1053. https://doi.org/10.1016/j.ijhe....
35.
Petrisek R, Hall J. Evaluation of a most probable number method for the enumeration of Legionella pneumophila from North American potable and non-potable water samples. J. Water Health. 2018;16(1):25–33. https://doi.org/10.2166/wh.201....
36.
Monteiro SN, Robalo AM, Santos RJ. Evaluation of Legiolert™ for the Detection of Legionella pneumophila and Comparison with Spread-Plate Culture and qPCR Methods. Curr Microbiol. 2021;78:1792–1797. https://doi.org/10.1007/s00284....
37.
Sartory DP, Spies K, Lange B, et al. Evaluation of a most probable number method for the enumeration of Legionella pneumophila from potable and related water samples. Lett Appl Microbiol. 2017;64(4):271–275. https://doi:10.1111/lam.12719.
38.
Le Chevallier MW. Monitoring distribution systems for Legionella pneumophila using Legiolert. AWWA Wat Sci. 2019;e1122. https://doi.org/10.1002/aws2.1....
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