Online first
Current issue
Archive
Special Issues
About the Journal
Publication Ethics
Anti-Plagiarism system
Instructions for Authors
Instructions for Reviewers
Editorial Board
Editorial Office
Contact
Reviewers
All Reviewers
2023
2022
2021
2020
2019
2018
2017
2016
General Data Protection Regulation (RODO)
RESEARCH PAPER
Comparison of filtering methods, filter processing and DNA extraction kits for detection of mycobacteria in water
1
Veterinary Research Institute, Brno, Czech Republic
Ann Agric Environ Med. 2015;22(3):429-432
KEYWORDS
ABSTRACT
Introduction and objective:
Mycobacteria have been isolated from almost all types of natural waters, as well as from man-made water distribution systems. Detection of mycobacteria using PCR has been described in different types of water; however, currently, there is no standardised protocol for the processing of large volumes of water.
Material and Methods:
In the present study, different filtering methods are tested and optimised for tap or river water filtration up to 10 L, as well as filter processing and DNA isolation using four commercially available kits.
Results:
The PowerWater DNA isolation kit (MoBio, USA), together with a kit used for soil and other environmental samples (PowerSoil DNA isolation kit, MoBio), had the highest efficiency. Filtration of 10 L of water and elution of the filter in PBS with the addition of 0.05% of Tween 80 is suggested.
Conclusions:
The described protocol for filter elution is recommended, and the use of the PowerWater DNA isolation kit for the highest mycobacterial DNA yield from water samples. The described protocol is suitable for parallel detection of mycobacteria using cultivation.
Mycobacteria have been isolated from almost all types of natural waters, as well as from man-made water distribution systems. Detection of mycobacteria using PCR has been described in different types of water; however, currently, there is no standardised protocol for the processing of large volumes of water.
Material and Methods:
In the present study, different filtering methods are tested and optimised for tap or river water filtration up to 10 L, as well as filter processing and DNA isolation using four commercially available kits.
Results:
The PowerWater DNA isolation kit (MoBio, USA), together with a kit used for soil and other environmental samples (PowerSoil DNA isolation kit, MoBio), had the highest efficiency. Filtration of 10 L of water and elution of the filter in PBS with the addition of 0.05% of Tween 80 is suggested.
Conclusions:
The described protocol for filter elution is recommended, and the use of the PowerWater DNA isolation kit for the highest mycobacterial DNA yield from water samples. The described protocol is suitable for parallel detection of mycobacteria using cultivation.
REFERENCES (18)
1.
Falkinham JO. Epidemiology of infection by nontuberculous myco-bacteria. Clin Microbiol Rev. 1996; 9(2): 177–215.
2.
Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, Holland SM, Horsburgh R, Huitt G, Iademarco MF, Iseman M, Olivier K, Ruoss S, von Reyn CF, Wallace RJ, Winthrop K. An official ATS/IDSA statement: Diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Resp Crit Care Med. 2007; 175(4): 367–416.
3.
Falkinham JO. Surrounded by mycobacteria: nontuberculous myco-bacteria in the human environment. J Appl Microbiol. 2009; 107(2): 356–367.
4.
Vaerewijck MJ, Huys G, Palomino JC, Swings J, Portaels F. Mycobacteria in drinking water distribution systems: ecology and significance for human health. FEMS Microbiol Rev. 2005; 29(5): 911–934.
5.
Whiley H, Keegan A, Giglio S, Bentham R. Mycobacterium avium complex – the role of potable water in disease transmission. J Appl Microbiol. 2012; 113(2): 223–232.
6.
Falkinham JO. Nontuberculous mycobacteria from household plumbing of patients with nontuberculous mycobacteria disease. Emerg Infect Dis. 2011; 17(3): 419–424.
7.
Nielsen SS, Toft N. A review of prevalences of paratuberculosis in farmed animals in Europe. Prev Vet Med. 2009; 88(1): 1–14.
8.
Rhodes G, Henrys P, Thomson BC, Pickup RW. Mycobacterium avium subspecies paratuberculosis is widely distributed in British soils and waters: implications for animal and human health. Environ Microbiol. 2013; 15(10): 2761–2774.
9.
Adrados B, Julian E, Codony F, Torrents E, Luquin M, Morato J. Prevalence and concentration of non-tuberculous mycobacteria in cooling towers by means of quantitative PCR: a prospective study. Curr Microbiol. 2011; 62(1): 313–319.
10.
Beumer A, King D, Donohue M, Mistry J, Covert T, Pfaller S. Detection of Mycobacterium avium subsp. paratuberculosis in drinking water and biofilms by quantitative PCR. Appl Environ Microbiol. 2010; 76(21): 7367–7370.
11.
Pickup RW, Rhodes G, Arnott S, Sidi-Boumedine K, Bull TJ, Weightman A, Hurley M, Hermon-Taylor J. Mycobacterium avium subsp. paratuberculosis in the catchment area and water of the river Taff in South Wales, United Kingdom, and its potential relationship to clustering of Crohn‘s disease cases in the city of Cardiff. Appl Environ Microbiol. 2005; 71(4): 2130–2139.
12.
Hruska K, Kaevska M. Mycobacteria in water, soil, plants and air: a review. Veterinarni Medicina. 2012; 57(12): 623–679.
13.
Slana I, Kralik P, Kralova A, Pavlik I. On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination. Int J Food Microbiol. 2008; 128(2): 250–257.
14.
Maunula L, Soderberg K, Vahtera H, Vuorilehto VP, von Bonsdorff CH, Valtari M, Laakso T, Lahti K. Presence of human noro- and adenoviruses in river and treated wastewater, a longitudinal study and method comparison. J Water Health. 2012; 10(1): 87–99.
15.
Chang CT, Wang LY, Liao CY, Huang SP. Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphism. Appl Environ Microbiol. 2002; 68(6): 3159–3161.
16.
Stinear T, Davies JK, Jenkin GA, Hayman JA, Oppedisano F, Johnson PDR. Identification of Mycobacterium ulcerans in the environment from regions in southeast Australia in which it is endemic with sequence capture-PCR. Appl Environ Microbiol. 2000; 66(8): 3206–3213.
17.
Jacobs J, Rhodes M, Sturgis B, Wood B. Influence of environmental gradients on the abundance and distribution of Mycobacterium spp. in a coastal lagoon estuary. Appl Environ Microbiol. 2009; 75(23): 7378–7384.
18.
Musial CE, Arrowood MJ, Sterling CR, Gerba CP. Detection of Cryptosporidium in water by using polypropylene cartridge filters. Appl Environ Microbiol. 1987; 53(4): 687–692.
| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
