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RESEARCH PAPER
Bacterial contamination of coffee and personal exposure to inhalable dust and endotoxin in primary coffee processing factories in Ethiopia
1
Department of Preventive Medicine, School of Public Health, College of Health Sciences, Addis Ababa University, Ethiopia
2
Department of Global Public Health and Primary Care, University of Bergen, Norway
3
Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Norway
Corresponding author
Magne Bråtveit
Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway
Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway
Ann Agric Environ Med. 2023;30(4):611-616
KEYWORDS
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Health effects of chemical pollutants in agricultural areas , including occupational and non-occupational effects of agricultural chemicals (pesticides, fertilizers) and effects of industrial disposal (heavy metals, sulphur, etc.) contaminating the atmosphere, soil and waterPrevention of occupational diseases in agriculture, forestry, food industry and wood industry
ABSTRACT
Introduction and objective:
Endotoxins from gram-negative bacteria might be released when the coffee cherries are processed and may cause respiratory health problems among workers in the coffee industry. The relationship between bacterial contamination and occupational exposure to endotoxin levels has not been thoroughly explored previously in primary coffee processing factories in Ethiopia, or elsewhere. The aim of this study was to characterize the level of personal endotoxin exposure and its relations with bacterial contamination of coffee cherries in such factories in Ethiopia.
Material and methods:
A cross-sectional study was conducted from March 2020 – February 2021 in 9 primary coffee processing factories in 3 regions in Ethiopia. A total of 180 personal air samples were collected to analyze workers’ exposure to inhalable dust and endotoxin. Correlation tests were performed to assess the relationship between total bacteria and endotoxin levels and between inhalable dust and endotoxin levels.
Results:
The geometric mean (GM) of personal inhalable dust exposure among machine room workers and hand pickers were 9.58 mg/m3 and 2.56 mg/m3, respectively. The overall GM of endotoxin exposure among machine room workers and hand pickers were 10,198 EU/m3 and 780 EU/m3, respectively. Gram-negative bacteria were found in all 54 coffee samples. The correlation between inhalable dust and endotoxin exposure was significant (r=0.80; P <0.01).
Conclusions:
About 92% of the samples from hand pickers and all samples from machine room workers exceeded the occupational exposure limit of 90 EU/m3 recommended by the Dutch Expert Committee on Occupational Standards. Prevention and control of bacterial contamination of the coffee in primary coffee processing are suggested to reduce endotoxin exposure that might cause respiratory health problems among coffee workers.
Endotoxins from gram-negative bacteria might be released when the coffee cherries are processed and may cause respiratory health problems among workers in the coffee industry. The relationship between bacterial contamination and occupational exposure to endotoxin levels has not been thoroughly explored previously in primary coffee processing factories in Ethiopia, or elsewhere. The aim of this study was to characterize the level of personal endotoxin exposure and its relations with bacterial contamination of coffee cherries in such factories in Ethiopia.
Material and methods:
A cross-sectional study was conducted from March 2020 – February 2021 in 9 primary coffee processing factories in 3 regions in Ethiopia. A total of 180 personal air samples were collected to analyze workers’ exposure to inhalable dust and endotoxin. Correlation tests were performed to assess the relationship between total bacteria and endotoxin levels and between inhalable dust and endotoxin levels.
Results:
The geometric mean (GM) of personal inhalable dust exposure among machine room workers and hand pickers were 9.58 mg/m3 and 2.56 mg/m3, respectively. The overall GM of endotoxin exposure among machine room workers and hand pickers were 10,198 EU/m3 and 780 EU/m3, respectively. Gram-negative bacteria were found in all 54 coffee samples. The correlation between inhalable dust and endotoxin exposure was significant (r=0.80; P <0.01).
Conclusions:
About 92% of the samples from hand pickers and all samples from machine room workers exceeded the occupational exposure limit of 90 EU/m3 recommended by the Dutch Expert Committee on Occupational Standards. Prevention and control of bacterial contamination of the coffee in primary coffee processing are suggested to reduce endotoxin exposure that might cause respiratory health problems among coffee workers.
ACKNOWLEDGEMENTS
The authors express their thanks for the funding received
from thee Norwegian Agency for Development Cooperation
(Norad) through the Norwegian Programme for Capacity
Building in Higher Education and Research for Development
(NORHED II), through the research project SAFEWORKERS
– Safe Work Conditions by Innovative Research and Education.
REFERENCES (37)
1.
Tamru S, Minten B. Value addition and farmers: Evidence from coffee in Ethiopia. PLoS One. 2023;18(1):e0273121. https://doi.org/10.1080/027868....
2.
Chauhan R, Hooda MS, Tanga AA. Coffee: The backbone of Ethiopian economy. Int J Econ Plants. 2015;2(1):018–022.
3.
Abaya SW, Bratveit M, Deressa W, et al. Reduced lung function among workers in primary coffee processing factories in Ethiopia: A Cross-Sectional Study. Int J Environ Res Public Health. 2018;15(11):2415. https://doi.org/10.3390/ijerph....
4.
Bratveit M, Abaya SW, Sakwari G, et al. Dust exposure and respiratory health among workers in primary coffee processing factories in Tanzania and Ethiopia. Front Public Health. 2021;9:730201. https://doi.org/10.3389/fpubh.....
5.
Abaya SW, Bratveit M, Deressa W, et al. Respiratory health among hand pickers in primary coffee-processing factories of Ethiopia. J Occup Environ Med. 2019;61(7):565–71. https://doi.org/10.1097/JOM.00....
6.
Anyfantis ID, Rachiotis G, Hadjichristodoulou C, et al. Bacterial endotoxins and their impact on respiratory system among Greek cotton industry workers. Int J Occup Environ Med. 2017;8(2):125–6. https://doi.org/10.15171/ijoem....
7.
Lusno MFD, Diyanah KC, Keman S. Lipopolysaccharides endotoxin-containing paddy dust leads to cross-shift lung function decline and respiratory complaints in paddy milling machine operators. Proceedings of the 2nd International Meeting of Public Health 2016, Nov 19–20; West Java, Indonesia. KnE Life Sci. 2018: 442–451. https://doi.org/10.18502/kls.v....
8.
Basinas I, Sigsgaard T, Kromhout H, et al. A comprehensive review of levels and determinants of personal exposure to dust and endotoxin in livestock farming. J Expo Sci Environ Epidemiol. 2015;25(2):123–37. https://doi.org/10.1038/jes.20....
9.
Wójcik-Fatla A, Mackiewicz B, Sawczyn-Domańska A, et al. Timber-colonizing gram-negative bacteria as potential causative agents of respiratory diseases in woodworkers. Int Arch Occup Environ Health. 2022;95(6):1179–93. https://doi.org/10.1007/s00420....
10.
Spierenburg EAJ, Smit LAM, Krop EJM, et al. Occupational endotoxin exposure in association with atopic sensitization and respiratory health in adults: Results of a 5-year follow-up. PLoS One. 2017;12(12):e0189097. https://doi.org/10.1371/journa....
11.
Paudyal P, Semple S, Gairhe S, et al. Respiratory symptoms and cross-shift lung function in relation to cotton dust and endotoxin exposure in textile workers in Nepal: a cross-sectional study. Occup Environ Med. 2015;72(12):870–6. https://doi.org/10.1136/oemed-....
12.
Ben Khedher S, Neri M, Guida F, et al. Occupational exposure to endotoxins and lung cancer risk: results of the ICARE Study. Occup Environ Med. 2017;74(9):667–79. https://doi.org/10.1136/oemed-....
13.
Hwang J, Golla V, Metwali N, et al. Inhalable and respirable particulate and endotoxin exposures in kentucky equine farms. J Agromedicine. 2020;25(2):179–89. https://doi.org/10.1080/105992....
14.
Arteaga VE, Mitchell DC, Matt GE, et al. Occupational exposure to endotoxin in PM2. 5 and pre-and post-shift lung function in California dairy workers. J Environ Prot 2015;6(05):552–65. https://doi.org/10.4236/jep.20....
15.
Van Der Eijk JAJ, Rommers JM, Van Hattum T, et al. Respiratory health of broilers following chronic exposure to airborne endotoxin. Res Vet Sci. 2022;147:74–82. https://doi.org/10.1016/j.rvsc....
16.
Ahmed MFE, Ramadan H, Seinige D, et al. Occurrence of extended-spectrum beta-lactamase-producing Enterobacteriaceae, microbial loads, and endotoxin levels in dust from laying hen houses in Egypt. BMC Vet Res. 2020;16(1):301. https://doi.org/10.1186/s12917....
17.
Roque K, Lim GD, Jo JH, et al. Epizootiological characteristics of viable bacteria and fungi in indoor air from porcine, chicken, or bovine husbandry confinement buildings. J Vet Sci. 2016;17(4):531–538. https://doi.org/10.4142/jvs.20....
18.
Wu B, Meng K, Wei L, et al. Seasonal fluctuations of microbial aerosol in live poultry markets and the detection of endotoxin. Front Microbiol. 2017; 8:551. https://doi.org/10.3389/fmicb.....
19.
Rappaport SM, Kupper LL. Quantitative exposure assessment. Stephen Rappaport, El Cerrito (CA), 2008.
20.
Shahhosseini E, Naddafi K, Nabizadeh R, et al. Endotoxin and Der p1 allergen levels in indoor air and settled dust in day-care centers in Tehran, Iran. J Environ Health Sci Eng. 2019;17(2):789–795. https://doi.org/10.1007/s40201....
21.
Li H, Hitchins VM, Wickramasekara S. Rapid detection of bacterial endotoxins in ophthalmic viscosurgical device materials by direct analysis in real-time mass spectrometry. Anal Chim Acta. 2016; 943:98–105. https://doi.org/10.1016/j.aca.....
22.
Shen C, Zhang Y. Enumeration of bacteria in broth suspension by spread and pour plating. In: Shen C, Zhang Y, editors. Food Microbiology Laboratory for the Food Science Student: A practical approach. Springer; 2023. p. 15–18.
23.
Maturin L, Peeler JT. Bacteriological Analytical Manual (BAM), Chapter 3: Aerobic Plate Count. US Food and Drug Administration: Silver Spring, USA. 2021. [access 2023.08.14] https://www.fda.gov/food/labor....
24.
Chiurazzi C, Motos-Galera A, Torres A. Early identification of ventilator-associated pneumonia causative pathogens: Focus on the value of Gram-stain examination. In: Vincent JL, editor. Annual Update in Intensive Care and Emergency Medicine. USA: Springer; 2015. p.3–14.
25.
Farokhi A, Heederik D. Smit L. Respiratory health effects of exposure to low levels of airborne endotoxin – a systematic review. Environ Health. 2018;17(14). https://doi.org/10.1186/s12940....
26.
Norwegian Labour Inspection Authority. Action and Limit Values: Regulations concerning action and limit values for physical and chemical agents in the working environment and classified biological agents. 2015 (access 2023.08.14). https://webhms.no/wp-content/t....
27.
Moen BE, Kayumba A, Sakwari G, et al. Endotoxin, dust and exhaled nitrogen oxide among hand pickers of coffee; a cross-sectional study. J Occup Med Toxicol. 2016;11(1):17. https://doi.org/10.1186/s12995....
28.
Abaya SW, Bratveit M, Deressa W, et al. Personal dust exposure and its determinants among workers in primary coffee processing in Ethiopia. Ann Work Expo Health. 2018;62(9):1087–95. https://doi.org/10.1093/annweh....
29.
Krug JD, Dart A, Witherspoon CL, et al. Revisiting the size selective performance of EPA’s high-volume total suspended particulate matter (Hi-Vol TSP) sampler. Aerosol Sci Technol. 2017;51(7):868–78.
30.
Tefera Y, Schlünssen V, Kumie A, et al. Personal inhalable dust and endotoxin exposure among workers in an integrated textile factory. Arch Environ Occup Health. 2020;75(7):415–21.
31.
Asgedom AA, Bratveit M, Schlünssen V, et al. Exposure to inhalable dust, endotoxin and formaldehyde in factories processing particleboards from eucalyptus trees in Ethiopia. Environmental and Occupational Health Practice. 2020;2(1). https://doi.org/10.1539/eohp.2....
32.
Abaya SW, Bratveit M, Deressa W, et al. Microbial contamination of coffee during postharvest on farm processing: A concern for the respiratory health of production workers. Arch Environ Occup Health. 2020;75(4):201–8. https://doi.org/10.1080/193382....
33.
Chhetry BSK, Dewangan KN, Mahato DK, et al. Endotoxins affecting human health during agricultural practices: An overview. Applied Chem. 2022;3(1):11–31. https://doi.org/10.3390/applie....
34.
Cyprowski M, Piotrowska M, Zakowska Z, et al. Microbial and endotoxin contamination of water-soluble metalworking fluids. Int J Occup Med Environ Health. 2007;20(4):365–71. https://doi.org/10.2478/v10001....
35.
Park DU, Ryu SH, Kim SB, et al. An assessment of dust, endotoxin, and microorganism exposure during waste collection and sorting. J Air Waste Manage Assoc. 2011;61(4):461–8. https://doi.org/10.3155/1047-3....
36.
Health and Safety Executive. General methods for sampling and gravimetric analysis of respirable, thoracic and inhalable aerosols. Health and Safety Executive, UK. https://www.hse.gov.uk/pubns/m... (access 2023.08.14).
37.
Ceballos D, King B, Beaucham C, et al. Comparison of a wipe method with and without a rinse to recover wall losses in closed face 37-mm cassettes used for sampling lead dust particulates. J Occup Environ Hyg. 2015;12(10):225–31. https://doi.org/10.1080/154596....
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