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RESEARCH PAPER
Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens – clinical submissions
1
Lab of Veterinary Public Health Protection, Faculty of Veterinary Medicine and Animal Science, University of Life Science,
Poznań, Poland
2
Faculty of Veterinary Medicine and Animal Science, University of Life Sciences, Poznań, Poland
3
Department of Biochemistry, Medical University of Silesia, School of Medicine in Katowice, Poland
4
Lab-Vet Laboratory, Tarnowo Podgórne, Poland
5
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, University of Science, Warsaw, Poland
Corresponding author
Michał Majewski
Lab of Veterinary Public Health Protection, Faculty of Veterinary Medicine and Animal Science, University of Life Sciences in Poznań, Słoneczna 1, 62-002, Złotniki, Poland
Lab of Veterinary Public Health Protection, Faculty of Veterinary Medicine and Animal Science, University of Life Sciences in Poznań, Słoneczna 1, 62-002, Złotniki, Poland
Ann Agric Environ Med. 2021;28(2):271-276
KEYWORDS
TOPICS
ABSTRACT
Introduction:
The article discusses the antimicrobial resistance of poultry-isolated bacteria in the Wielkopolska region of Poland.
Material and methods:
From August 2014 – June 2016, antibiotic resistance screening tests were performed involving 4,496 samples of Escherichia coli and 84 samples of Klebsiella spp., and the following antibiotics: amoxicillin, amoxicillin with clavulanic acid, colistin, doxycycline, enrofloxacin, florfenicol, neomycin, norfloxacin, spectinomycin, and trimethoprim with sulfamethoxazole. The research used broth the microdilution method and CLSI standards.
Results:
During the investigation period of 22 months a growing percentage of E. coli isolates showed antibiotic resistance to amoxicillin, amoxicillin with clavulanic acid, colistin, enrofloxacin, neomycin, norfloxacin, spectinomycin, and trimethoprim with sulfamethoxazole. Resistance to doxycycline and florfenicol decreased. The most efficient antibiotics against E. coli were colistin (84.64 %), neomycin (80.62 %), and amoxicillin with clavulanic acid (73.05 %). Klebsiella samples were the most susceptible to neomycin (85.71 %), colistin (84.52 %), and trimethoprim with sulfamethoxazole (73.81 %).
Conclusions:
Antibiotic resistance of pathogenic micro-organisms, such as Escherichia coli and Klebsiella spp., is a serious problem both for poultry producers and for public health protection. Low efficiency of numerous antibiotic groups forces reflection on limiting the use of medicines in food-producing animals.
The article discusses the antimicrobial resistance of poultry-isolated bacteria in the Wielkopolska region of Poland.
Material and methods:
From August 2014 – June 2016, antibiotic resistance screening tests were performed involving 4,496 samples of Escherichia coli and 84 samples of Klebsiella spp., and the following antibiotics: amoxicillin, amoxicillin with clavulanic acid, colistin, doxycycline, enrofloxacin, florfenicol, neomycin, norfloxacin, spectinomycin, and trimethoprim with sulfamethoxazole. The research used broth the microdilution method and CLSI standards.
Results:
During the investigation period of 22 months a growing percentage of E. coli isolates showed antibiotic resistance to amoxicillin, amoxicillin with clavulanic acid, colistin, enrofloxacin, neomycin, norfloxacin, spectinomycin, and trimethoprim with sulfamethoxazole. Resistance to doxycycline and florfenicol decreased. The most efficient antibiotics against E. coli were colistin (84.64 %), neomycin (80.62 %), and amoxicillin with clavulanic acid (73.05 %). Klebsiella samples were the most susceptible to neomycin (85.71 %), colistin (84.52 %), and trimethoprim with sulfamethoxazole (73.81 %).
Conclusions:
Antibiotic resistance of pathogenic micro-organisms, such as Escherichia coli and Klebsiella spp., is a serious problem both for poultry producers and for public health protection. Low efficiency of numerous antibiotic groups forces reflection on limiting the use of medicines in food-producing animals.
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