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BRIEF COMMUNICATION
Neoehrlichia mikurensis: an emerging pathogen in Southeastern Poland – prevalence in Ixodes ricinus ticks and phylogenetic characterization
1
Department of Biology and Parasitology, Medical University, Lublin, Poland
2
Laboratoire de Santé Animale, Anses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Maisons-Alfort, France
3
Clinic for Lyme Borreliosis and Other Tick-Borne Diseases, Department of Prevention of Rabies and Other Infectious Diseases, Pasteur Institute, Novi Sad, Serbia
4
Department of Microbiology with Parasitology and Immunology, Faculty of Medicine, University of Novi Sad, Serbia
5
Diagnostics and Laboratory Research Task Force, Balkan Association for Vector-Borne Diseases, Novi Sad, Serbia
6
Department of Animal Production and Food Safety, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania
Corresponding author
Zbigniew Zając
Department of Biology and Parasitology, Medical University of Lublin, Poland, Radziwiłłowska 11, 20-080, Lublin, Poland
Department of Biology and Parasitology, Medical University of Lublin, Poland, Radziwiłłowska 11, 20-080, Lublin, Poland
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Neoehrlichia mikurensis is an emerging pathogen increasingly detected in ticks, animals, and humans. The study aimed to investigate the presence and phylogenetic diversity of Ixodes ricinus ticks in the Subcarpathian Region.
Material and Methods:
Of the 412 ticks collected from forest and meadow habitats, 240 specimens underwent molecular analysis for presence of N. mikurensis using microfluidic real-time PCR. Selected amplicons were sequenced to preform phylogenetic analysis of the groEL gene.
Results:
N. mikurensis infection was confirmed in 6.35% of female and 2.38% of male ticks, with no significant differences in the prevalence between habitats. Phylogenetic analysis revealed a low genetic diversity N. mikurensis.
Conclusions:
The findings confirm the infection of I. ricinus ticks with N. mikurensis in the studied region. The studied N. mikurensis groEL gene sequences showed low genetic variation, identical to other populations in Poland and Europe.
Neoehrlichia mikurensis is an emerging pathogen increasingly detected in ticks, animals, and humans. The study aimed to investigate the presence and phylogenetic diversity of Ixodes ricinus ticks in the Subcarpathian Region.
Material and Methods:
Of the 412 ticks collected from forest and meadow habitats, 240 specimens underwent molecular analysis for presence of N. mikurensis using microfluidic real-time PCR. Selected amplicons were sequenced to preform phylogenetic analysis of the groEL gene.
Results:
N. mikurensis infection was confirmed in 6.35% of female and 2.38% of male ticks, with no significant differences in the prevalence between habitats. Phylogenetic analysis revealed a low genetic diversity N. mikurensis.
Conclusions:
The findings confirm the infection of I. ricinus ticks with N. mikurensis in the studied region. The studied N. mikurensis groEL gene sequences showed low genetic variation, identical to other populations in Poland and Europe.
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