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BRIEF COMMUNICATION
Cryptosporidium spp. in dogs and cats in Poland
1
Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Division of Parasitology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Poland
Ann Agric Environ Med. 2021;28(2):345-347
KEYWORDS
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industry
ABSTRACT
Introduction:
Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats.
Objective:
The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland
Material and Methods:
A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene.
Results:
Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats.
Conclusions:
This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.
Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats.
Objective:
The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland
Material and Methods:
A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene.
Results:
Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats.
Conclusions:
This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.
ACKNOWLEDGEMENTS
This research was supported by statutory research and development activity founds by Minister of Science and Higher Education assigned to Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences,
Poland.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
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