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RESEARCH PAPER
Effects of sauna bathing on stress-related genes expression in athletes and non-athletes
1
Gdansk University of Physical Education and Sport, Department of Life Sciences, Gdansk, Poland
2
Jan Kochanowski University in Kielce, Department of Medicine and Health, Kielce, Poland
3
Gdansk University of Physical Education and Sport, Department of Management in Sport, Gdańsk, Poland
4
Pedagogical University, Department of Special Pedagogy, Kraków, Poland
5
Old Polish University, Kielce, Poland
Corresponding author
Małgorzata Żychowska
Gdansk University of Physical Education and Sport, Department of Life Sciences, Gdansk, Poland
Gdansk University of Physical Education and Sport, Department of Life Sciences, Gdansk, Poland
Ann Agric Environ Med. 2017;24(1):104-107
KEYWORDS
ABSTRACT
Introduction and objective:
Heat stress induces the expression of genes encoding heat-shock proteins and immune response mediators. The aim of this study was to determine the differences in the expression of genes encoding heat-shock proteins 70 kDa and27 kDa, interleukin 6, interleukin 10and C-reactive protein, between athletes and non-athletes after sauna bathing.
Material and Methods:
Athletes (n=9) and non-athletes (n=9) were exposed to a Finnish sauna twice during one session at a temperature of 98.2 °C and humidity of 10% ± 2%, with a 5 min break for cooling down under a shower. The groups did not differ in terms of age, height or body mass. Blood samples were taken before and after sauna exposure in order to assess gene expression, using reverse transcription polymerase chain reaction.
Results:
Differences were observed in leukocyte mRNA levels of tested genes between athletes and non-athletes. In the non-athlete group, all the tested genes were expressed at higher levels as a response to the same heat challenge.
Conclusions:
It appears that expression of stress-related genes induced by heat stress is dependent on the level of physical activity
Heat stress induces the expression of genes encoding heat-shock proteins and immune response mediators. The aim of this study was to determine the differences in the expression of genes encoding heat-shock proteins 70 kDa and27 kDa, interleukin 6, interleukin 10and C-reactive protein, between athletes and non-athletes after sauna bathing.
Material and Methods:
Athletes (n=9) and non-athletes (n=9) were exposed to a Finnish sauna twice during one session at a temperature of 98.2 °C and humidity of 10% ± 2%, with a 5 min break for cooling down under a shower. The groups did not differ in terms of age, height or body mass. Blood samples were taken before and after sauna exposure in order to assess gene expression, using reverse transcription polymerase chain reaction.
Results:
Differences were observed in leukocyte mRNA levels of tested genes between athletes and non-athletes. In the non-athlete group, all the tested genes were expressed at higher levels as a response to the same heat challenge.
Conclusions:
It appears that expression of stress-related genes induced by heat stress is dependent on the level of physical activity
REFERENCES (28)
1.
Sawka MN, Leon LR, Montain SJ, Sonna LA. Integrated physiological mechanisms of exercise performance, adaptation, and maladaptation to heat stress. ComparPhysiol 2011; 4: 1883–928.
2.
Pilch W, Szygula Z, Tyka AK, Palka T, Tyka A, Cison T, et al. Disturbances in pro-oxidant-antioxidant balance after passive body overheating and after exercise in elevated ambient temperatures in athletes and untrained men. PLoS One 2014; 20: 9(1).
3.
Rutkowski R, Pancewicz SA, Skrzydlewska E, Hermanowska-Szpakowicz T. Biology of nucleartranscriptionfactor NF-kB. Allergy Asthma Immunol 2005; 10: 125–31.
4.
Szołtysek K, Janus P, Widłak P. The NFkB dependent cellular signaling pathway and its interference with p53 and HSF-1 depentent pathways. Adv Cell Biol 2011; 38: 159–75.
5.
Perkins ND. Integrating cell-signalling pathways with NF-kappaB and IKK function. NatRev Mol Cell Biol 2007; 8: 49–62.
6.
Morimoto RI. Regulation of the heat shock transcriptional response; cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev 1998; 2: 3788–96.
7.
Radák Z, Naito H, Kaneko T, Tahara S, Nakamoto H, Takahashi R, et al. Exercise training decreases DNA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle.Pflugers Arch 2002; 445(2): 273–8.
8.
Kregel KCJ. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. Appl. Physiol 2002; 92: 2177–86.
9.
Arya R, Mallik M, Lakhotia SC. Heat shock genes-integrating cell survival and death. J Biosci. 2007; 32: 595–610.
10.
Feng X, Bonni S, Riabowol K. HSP70 induction by ING proteins sensitizes cells to tumor necrosis factor alpha receptor-mediated apoptosis. Mol Cell Biol. 2006; 24: 9244–55.
11.
Dudeja V, Vickers SM, Saluja AK. The role of heat shock proteins in gastrointestinal diseases. Gut 2009; 58: 1000–9.
12.
Ferat-Osorio E, Sánchez-Anaya A, Gutiérrez-Mendoza M, Boscó-Gárate I, Wong-Baeza I, Pastelin-Palacios R, et al. Heat shock protein 70 down-regulates the production of toll-like receptor-induced pro-inflammatory cytokines by a heat shock factor-1/constitutive heat shock element-binding factor-dependent mechanism. J Inflamm 2014; 12: 11:19.
13.
Radom-Aizik S, Zaldivar F Jr, Leu SY, Galassetti P, Cooper DM. Effects of 30 min of aerobic exercise on gene expression in human neutrophils. J ApplPhysiol. 2008; 104: 236–43.
14.
Neubauer O, Sabapathy S, Lazarus R, Jowett JBM, Desbrow B, Peake Cameron-Smith JM, et al. Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress. J ApplPhysiol. 2013; 114(12): 1677–88.
15.
Büttner P, Mosig S, Lechtermann A, Funke H, Mooren FC. Exercise affects the gene expression profiles of human white blood cells. J Appl. Physiol. 2006; 102(1): 26–36.
16.
Ernst E, Pecho E, Wirz P, Saradeth T. Regular sauna bathing and the incidence of common colds. Ann Med 1990; 22: 225–7.
17.
Dugué B, Leppänen E, Graé-Sbeck R. Effects of thermal stress (sauna + swimming in ice-cold water) in man on the blood concentration and production of pro-inflammatory cytokines and stress hormones.Pathophysiol. 1998; 5: 149–58.
18.
Pilch W, Pokora I, Szyguła Z, Pałka T, Pilch P, Cisoń T, et al. Effect of a single finnish sauna session on white blood cell profile and cortisol levels in athletes and non-athletes. J Hum Kinet. 2013; 39: 127–35.
19.
Chomczyński P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162: 156–9.
20.
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. NatProtoc. 2008; 3: 1101–8.
21.
Sawka MN, Leon LR, Montain SJ, Sonna LA. Integrated physiological mechanisms of exercise performance, adaptation, and maladaptation to heat stress. ComparPhysiol. 2011; 4: 1883–928.
22.
Chen ZC. WuWS, Lin MT, Hsu CC. Protective effect of transgenic expression of porcine heat shock protein 70 on hypothalamic ischemic and oxidative damage in a mouse model of heatstroke. BMC Neurosci. 2009; 3: 10–11.
23.
Pizurki L, Polla BS. cAMP modulates stress protein synthesis in human monocytes-macrophages. J Cell Physiol. 1994; 161: 169–177.
24.
Jacquier-Sarlin MR, Jornot L, Polla BS. Differential expression and regulation of hsp70 and hsp90 by phorbol esters and heat shock. J Biol Chem. 1995; 270: 14094–9.
25.
Polla BS, Cossarizza A. Stress proteins in inflammation. EXS 2002; 77: 375–91.
26.
Oehler R, Pusch E, Zellner M, Dungel P, Hergovics N, Homoncik M, et al. Cell type–specific variations in the induction of hsp70 in human leukocytes by feverlike whole body hyperthermia. Cell Stress Chap. 2001; 6: 306–15.
27.
Lam KK, Cheng PY, Lee YM, Liu YP, Ding C, Liu WH, et al. The role of heat shock protein 70 in the protective effect of YC-1 on heat stroke rats. Eur J Pharmacol. 2013; 15: 67–73.
28.
Moro AS, PolitiOkoshi M, Padovani CR, Okoshi K Doppler echocardiography in athletes from different sports . Med SciMonit. 2013; 19: 187–193.
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