RESEARCH PAPER
Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse
 
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1
University of Veterinary Medicine and Pharmacy, Kosice, Slovakia
 
2
Faculty of Medicine, Department of Medical and Clinical Microbiology, P. J. Safarik University, Kosice, Slovakia
 
3
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
 
4
Slovak University of Agriculture, Nitra, Slovakia
 
 
Corresponding author
Vladimir Kmet   

Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
 
 
Ann Agric Environ Med. 2012;19(1):75-77
 
KEYWORDS
ABSTRACT
The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
 
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ISSN:1232-1966
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