Proliferation of cells and expression of RARs, RXRs and HPV viral E6 and E7 proteins in cervical cancer cell lines after treatment with ATRA.

Magdalena Myga-Nowak 1, Joanna Pacholska-Bogalska 2, Wojciech Kwasniewski 2, Anna Kwasniewska 2, Anna Gozdzicka-Jozefiak 2
1 - Department of Molecular Virology, Umultowska 89, 61-614 Poznan, Poland.
2 -
Ann Agric Environ Med
2011; 18 (1):
ICID: 956893
All-trans retinoic acid (ATRA) is considered to be a potential chemotherapeutic drug due to its capability to regulate cell growth and differentiation. The effects of ATRA on the proliferation of cells and gene regulation are mediated by retinoid receptors (RAR and RXR), which belong to the nuclear receptor superfamily of ligand- inducible transcription factors. ATRA can act either as a growth inhibitor or growth promoter, according to the functional state of retinoic receptors. Thus, we have established the effect of ATRA on the proliferation of cervical cancer cells line HeLa and CaSki and expression of retinoids receptors as well as the viral HPV oncogenic proteins E6 and E7. ATRA had no effect on proliferation CaSki cells, but it stimulated the growth of HeLa cells, which depended on the incubation time and the concentration of ATRA in cell culture. The overexpression of RAR alpha in HeLa cells after the administration of 10-7 mM ATRA was also observed 72 hours, and the decrease of CaSki by 60-90 percent. In the study of cervical cancer cell lines, the very low levels of other endogenous RAR and RXR receptors were observed. ATRA does not repress the expression of two viral oncoproteins E6 and E7 HPV16/18, which play a key role in carcinogenesis of the cervix. Our results support the suggestions that the cell response to vitamin A, and other retinoids in the diet, may depend on cell type, and that the cancer cells are differentially resistant to retinoids. Thus, despite the important biological functions of retinoids, the effects of retinoids in a supplementation in supra-physiological doses as well as their physiological action are difficult to define.
PMID 21736279 - click here to show this article in PubMed

Recommend this article to:


Related articles in IndexCopernicus™
     ATRA [4 related records]
     cervical cancer cell lines [0 related records]
     proliferation [122 related records]
     RXRs RARs expression [0 related records]


Related articles

A LIU , X LI ,
 Effects of HMGB2 silencing on proliferation´...
 Objective To investigate the effect of HMGB2 silencing on proliferation´╝î apoptosis, invasion and migration of breast cancer cell line MDA-MB-231. Methods The lentiviral vector was constructed by cloning the oligonucleotides targeting HMGB2 ...
 Effect of BML-210 on the proliferation´╝î ap...
 Objective To investigate the effect of histone acetylation enzyme inhibitor´╝łBML-210´╝ë on the proliferation´╝î apoptosis and cell cycle of glioma U251 cells. Methods U251 cells were treated with 0´╝î 5´╝î 10 and 20 ╬╝mol´╝ĆL BML-210. The prol...
 Experimental study of toxoflavin on human no...
 Objective To investigate the inhibitory effects of toxoflavin on proliferation´╝î apoptosis and migration of non-small cell lung cancer A549 cells. Methods The cultured A549 cells were treated with toxoflavin´╝ł0.5´╝î 0.375´╝î 0.25´╝î 0.125 ╬╝m...