Molecular and serological diagnosis of Borrelia burgdorferi infection among patients with diagnosed erythema migrans.

Maciej Kondrusik 1, Sambor Grygorczuk 2, Bogumila Skotarczak 2, Beata Wodecka 2, Anna Rymaszewska 2, Slawomir Pancewicz 2, Joanna Zajkowska 2, Renata Swierzbinska 2, Teresa Hermanowska-Szpakowicz 2
1 - Department of Infectious Diseases and Neuroinfections, Medical University, Zurawia 14, 15-540 Bialystok, Poland; mkodrusik@poczta.onet.pl.
2 -
Ann Agric Environ Med
2007; 14 (2):
ICID: 778146
Article type: Original article
 
 
The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Bialystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the first at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.
PMID 18247452 - click here to show this article in PubMed
STATS

Recommend this article to:

Name:
E-mail:
From:
Language:


Related articles in IndexCopernicus™
     borreliosis [22 related records]
     erythema migrans [11 related records]
     Borrelia burgdorferi [49 related records]
     Nested PCR [21 related records]
     ELISA [240 related records]



 

Related articles

M Tokarska-Rodak, M Kozioł-Montewka,
Analysis of nanomechanical properties of Borrelia ...
Background: Atomic force microscopy (AFM) is an experimental technique which recently has been used in biology, microbiology, and medicine to investigate the topography of surfaces and in the evaluation of mechanical properties of cells. The aim of t...
ABSTRACT
M Bhuyan , S Sharma,
Emergence of Anti-Citrullinated Protein in the Ear...
Introduction: Rheumatoid arthritis (RA) is one of the most common systemic autoimmune diseases. The aetiology of the disease, which affects up to 12 % of the world population, is unknown. This poor prognosis has led to an emphasis on early diagnos...
ABSTRACT
R Ataee, M Kahani,
Staphylococcal Enterotoxin A Detection from Rheuma...
Direct detection of microbial super antigens in synovial fluid of patients with rheumatoid arthritis may be able to guide to the design of cost-effective therapies. The purpose of this study was to assess the existence of Staphylococcal enterotoxin A...
ABSTRACT