A total of 50 samples of poultry feed mixtures of Slovak origin were analysedfor fumonisin B(1) and B(2) (FB(1), FB(2)) and moniliformin (MON) using SAX-clean up procedure beingdetected by high pressure liquid chromatography with mass spectrometry (HPLC-MS) and diode array detection(HPLC-DAD), respectively. The samples were also simultaneously investigated for Fusarium species occurrence,and for the capability of Fusarium isolates recovered to produce FB(1) and MON in vitro. FB1 was detectedin 49 samples (98 %) in concentrations ranging from 43 to 798 microg x kg(-1), and FB(2) in 42 samples(84 %) in concentrations ranging from 26 to 362 microg x kg(-1). MON was detected in 26 samples (52 %)in concentrations that ranged from 42 to 1,214 microg x kg(-1). Only two Fusarium populations were encountered,namely F. proliferatum and F. subglutinans, of which the former was the most dominant and frequent. All86 F. proliferatum isolates tested for FB1-production ability proved to be producers of the toxin althoughnone of them produced MON. On the contrary, MON production was observed in a half out of 16 F. subglutinansisolates tested, yet no FB1 production was detected in this case. Despite the limited number of samplesinvestigated during this study, it is obvious that poultry feed mixtures may represent a risk from atoxicological point of view and should be regarded as a potential source of the Fusarium mycotoxins incentral Europe. This is the first reported study dealing with fumonisin and moniliformin contaminationof poultry feeds from Slovakia.