The induction of micronuclei in bovine lymphocytes by exposure to benzene andS-9 mix.
Katarina Sivikova 1, Elena Piesova 2 1 - 2 - Department of Genetics, University of Veterinary Medicine, Komenskeho 73, 04181 Kosice, Slovak Republic. firstname.lastname@example.org Ann Agric Environ Med 2003; 10 (2): ICID: 142699
Benzene is a widespread human carcinogen, inducing leukaemia and hematotoxicity.It has been shown to be a multi-organ carcinogen in animals. The effect of benzene was studied usinginduction of micronuclei (MN) in whole blood lymphocytes cultures after treatment with different concentrationsof benzene (5, 10, 50, 100, 500 and 1000 microM) with and without metabolic activation (S-9 mix). A significantelevation in the induction of micronuclei was found after application of benzene at doses of 50 and 100microM in both donors. Treatment of bovine lymphocytes did not result in the induction of micronucleiin a dose-dependent manner. The addition of an external metabolic factor (10 % S-9 mix for 2 h) in bloodcultures treated with benzene indicated an increase of the genotoxic activity of benzene (at concentrationsranging from 10-100 microM).