The induction of micronuclei in bovine lymphocytes by exposure to benzene andS-9 mix.

Katarina Sivikova 1, Elena Piesova 2
1 -
2 - Department of Genetics, University of Veterinary Medicine, Komenskeho 73, 04181 Kosice, Slovak Republic. piesova@uvm.sk
Ann Agric Environ Med
2003; 10 (2):
ICID: 142699
 
 
Benzene is a widespread human carcinogen, inducing leukaemia and hematotoxicity.It has been shown to be a multi-organ carcinogen in animals. The effect of benzene was studied usinginduction of micronuclei (MN) in whole blood lymphocytes cultures after treatment with different concentrationsof benzene (5, 10, 50, 100, 500 and 1000 microM) with and without metabolic activation (S-9 mix). A significantelevation in the induction of micronuclei was found after application of benzene at doses of 50 and 100microM in both donors. Treatment of bovine lymphocytes did not result in the induction of micronucleiin a dose-dependent manner. The addition of an external metabolic factor (10 % S-9 mix for 2 h) in bloodcultures treated with benzene indicated an increase of the genotoxic activity of benzene (at concentrationsranging from 10-100 microM).
PMID 14677922 - click here to show this article in PubMed
STATS

Recommend this article to:

Name:
E-mail:
From:
Language:


Related articles in IndexCopernicus™
     Micronuclei, Chromosome-Defective - drug effects [35 related records]
     Benzene - administration & dosage [11 related records]



 

Related articles

 Mąka, E Maćkiw,
Occurrence and antimicrobial resistance of Salmone...
Introduction and objectives. Antimicrobial resistance of pathogenic bacteria can result in therapy failure, increased hospitalization, and increased risk of death. In Poland, Salmonella spp. is a major bacterial agent of food poisoning. The ma...
ABSTRACT
L Kapka-Skrzypczak, K Sawicki,
Cholinesterase activity in blood and pesticide pre...
Introduction. On the contrary to the adult population exposed to pesticides, mostly on occupational basis, rural children are mostly exposed to pesticides deposited in the environment. However, even this constant, distributed in time exposure to low ...
ABSTRACT
E Cisak, A Wójcik-Fatla,
Spiroplasma – an emerging arthropod-borne pathog...
Spiroplasma is a genus of wall-less, low-GC, small Gram-positive bacteria of the internal contractile cytoskeleton, with helical morphology and motility. The genus is classified within the class Mollicutes. Spiroplasma / host interactio...
ABSTRACT