Cytotoxicity induced by cypermethrin in Human Neuroblastoma Cell Line SH-SY5Y
Grzegorz Raszewski 1, Marta Kinga Lemieszek 2, Krzysztof Łukawski 3 1 - Department of Physiopathology, Institute of Rural Health, Lublin, Poland 2 - Department of Medical Biology, Institute of Rural Health, Lublin, Poland 3 - Department of Physiopathology, Institute of Rural Health, Lublin, Poland Ann Agric Environ Med 2016; 23 (1): ICID: 1196863 Article type: Original article
The purpose of this study was to evaluate the cytotoxic potential of Cypermethrin (CM) on cultured human Neuroblastoma SH-SY5Y cells. SH-SY5Y cells were treated with CM at 0–200µM for 24, 48, and 72 h, in vitro. It was found that CM induced the cell death of Neuroblastoma cells in a dose- and time-dependent manner, as shown by LDH assays. Next, some aspects of the process of cell death triggered by CM in the human SH-SY5Y cell line were investigated. It was revealed that the pan-caspase inhibitor Q-VD-OPh, sensitizes SH-SY5Y cells to necroptosis caused by CM. Furthermore, signal transduction inhibitors PD98059, SL-327, SB202190, SP600125 failed to attenuate the effect of the pesticide. Finally, it was shown that inhibition of TNF-a by Pomalidomide (PLD) caused statistically significant reduction in CM-induced cytotoxicity. Overall, the data obtained suggest that CM induces neurotoxicity in SH-SY5Y cells by necroptosis.
DOI: 10.5604/12321966.1196863 PMID 27007526 - click here to show this article in PubMed