Cytotoxicity induced by cypermethrin in Human Neuroblastoma Cell Line SH-SY5Y

Grzegorz Raszewski 1, Marta Kinga Lemieszek 2, Krzysztof Łukawski 3
1 - Department of Physiopathology, Institute of Rural Health, Lublin, Poland
2 - Department of Medical Biology, Institute of Rural Health, Lublin, Poland
3 - Department of Physiopathology, Institute of Rural Health, Lublin, Poland
Ann Agric Environ Med
2016; 23 (1):
ICID: 1196863
Article type: Original article
The purpose of this study was to evaluate the cytotoxic potential of Cypermethrin (CM) on cultured human Neuroblastoma SH-SY5Y cells. SH-SY5Y cells were treated with CM at 0–200µM for 24, 48, and 72 h, in vitro. It was found that CM induced the cell death of Neuroblastoma cells in a dose- and time-dependent manner, as shown by LDH assays. Next, some aspects of the process of cell death triggered by CM in the human SH-SY5Y cell line were investigated. It was revealed that the pan-caspase inhibitor Q-VD-OPh, sensitizes SH-SY5Y cells to necroptosis caused by CM. Furthermore, signal transduction inhibitors PD98059, SL-327, SB202190, SP600125 failed to attenuate the effect of the pesticide. Finally, it was shown that inhibition of TNF-a by Pomalidomide (PLD) caused statistically significant reduction in CM-induced cytotoxicity. Overall, the data obtained suggest that CM induces neurotoxicity in SH-SY5Y cells by necroptosis.
DOI: 10.5604/12321966.1196863
PMID 27007526 - click here to show this article in PubMed

Recommend this article to:


Related articles in IndexCopernicus™
     mechanism [162 related records]
     necroptosis [3 related records]
     Cell Death [340 related records]
     Cypermethrin [15 related records]


Related articles

M Angelina Thanga Ajisha, K Rajagopal,
Fluoride removal study using pyrolyzed Delonix reg...
Applicability of the new material pyrolyzed Delonix regia pod carbon for the removal of excess fluoride ions from the water that harm the human and the environment was studied. The adsorbent was characterized both physically and chemically. Surface c...
E Wiśnik, M Ryksa,
PARP1 inhibitors: contemporary attempts at their u...
Current cancer therapies are based mainly on the use of compounds that cause DNA damage. Unfortunately, even the combination therapies do not give rewarding effects, due to the high efficiency of DNA damage repair mechanisms in tumor cells. Therefore...
R Nath , B Mahajon,