Serological and molecular evidence of Coxiella burnetii in samples from humans and animals in China

Heba S. El-Mahallawy  1, Patrick Kelly 2, Jilei Zhang 3, Yi Yang 4, Lanjing Wei 4, Lili Tian 5, Weixing Fan 5, Zhenwen Zhang 6, Chengming Wang 7
1 - Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Jiangsu, China; Department of Animal Hygiene, Zoonoses and Animal Behavior and Management, Faculty of Veterinary Medicine, Suez Canal University, Egypt
2 - Ross University School of Veterinary Medicine, West Indies
3 - Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou 225009, Jiangsu, China
4 - Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Jiangsu, China
5 - Laboratory of Zoonosis, China Animal Health and Epidemiology Centre, Shandong, China
6 - Yangzhou University College of Medicine, Yangzhou 225009, Jiangsu, China
7 - Yangzhou University College of Medicine, Jiangsu, China
Ann Agric Environ Med
2016; 23 (1):
ICID: 1196859
Article type: Original article
 
 
Introduction. Coxiella burnetii is the agent of Q fever, a worldwide zoonosis. To add to the available knowledge of the disease in China, C. burnetti infections were investigated in convenience samples from five animal species and humans from Yangzhou, Jiangsu province, eastern China.
Materials and methods. Commercial ELISA kits were used to detect antibodies to phase I and II C. burnetii. A FRET-qPCR targeting the outer membrane protein com1 gene was also developed to detect C. burnetii DNA in blood samples from animals and humans, and bovine milk samples.
Results. Seropositive cattle (44/150; 29%), goats (33/150; 22%), humans (45/180; 25%) and pigs (4/130; 3%) were found, while dogs (0/136; 0%) and cats (0/140; 0%) were seronegative. Seropositivity in humans was associated with increasing age, but there was no gender difference. DNA was amplified from two milk samples (2/150, 1.3%), while none of the blood samples were positive. The sequences of the obtained amplicons were identical to those of the com1 gene of the universal C. burnetii RSA 493 strain and other stains from China.
Conclusions. The findings indicaten that C. burnetii is endemic in Yangzhou, China, and therefore human and animal health workers should be aware of the possibility of infections and the occurrence of outbreaks of Q fever.
DOI: 10.5604/12321966.1196859
PMID 27007523 - click here to show this article in PubMed
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