Activity of MMP-2, MMP-8 and MMP-9 in serum
as a marker of progression of alcoholic liver disease in people from Lublin Region, eastern Poland

Andrzej Prystupa 1, Anna Boguszewska-Czubara 2, Agnieszka Bojarska-Junak 3, Anna Toruń-Jurkowska 4, Jacek Roliński 5, Wociech Załuska 6
1 - Department of Internal Medicine, Medical University of Lublin, Poland
2 - Department of Medical Chemistry, Medical Univeristy of Lublin, Poland
3 - Department of Clinical Immunology, Medical University of Lublin, Poland
4 - Department of Mathematics and Medical Biostatistics, Medical University of Lublin, Poland
5 - Department of Clinical Immunology, Medical University of Lublin, Poland
6 - Department of Nephrology, Medical University of Lublin, Poland
Ann Agric Environ Med
2015; 22 (2):
ICID: 1152088
Article type: Original article
In alcoholic liver cirrhosis, normal liver cells are replaced by scar tissue (fibrosis). Liver fibrosis is a dynamic process in which activated hepatic stellate cells are involved in the synthesis of matrix proteins and the regulation of matrix degeneration. The aim of the presented study was to assess the usefulness of MMP-2, MMP-8 and MMP-9 as diagnostic markers of alcoholic liver disease. Sixty patients with alcoholic liver cirrhosis were randomly enrolled during hospitalization in departments of hospitals from the Lublin Region in eastern Poland. The stage of cirrhosis was estimated according to Child-Turcotte-Pugh criteria (Child-Pugh score) as P- Ch A, P-Ch B, P-Ch C. The control group consisted of 10 healthy persons without liver disease, who did not drink alcohol. Additionally, a group of alcoholics without liver cirrhosis was included in the study. Blood sample were obtained, and after centrifuge, serum was collected for further analysis. The activity of MMP-2, MMP-8 and MMP-9 in the blood plasma of the patients and the control group were measured by using the sandwich enzyme immunoassay technique with commercially available quantitative ELISA test kits. Activity of MMP-2, MMP-8 and MMP-9 in patients with liver cirrhosis were increased gradually according to Child-Pugh stages. The activity of MMP-2, MMP-8, MMP-9 were the highest in patients with liver cirrhosis stage C. MMP-2, MMP-8, MMP-9 concentrations in the people with liver cirrhosis (stage C) were significantly increased compared to controls. A significant difference were observed between activity MMP-2 in control group, alcoholics without liver cirrhosis, and those with liver cirrhosis (stages A, B, C according Child-Pugh score). MMP-2, MMP-8 and MMP-9 may be markers of alcoholic liver cirrhosis in the alcoholics. Elevated levels of MMP-2, MMP-8 and MMP-9 in the alcoholic patients indicated that cirrhosis has developed. The most sensitive is MMP-2, because the activity of this parameter is increased in all liver cirrhosis stages. MMP-8 and MMP-9 activity were significantly elevated only in serum patients with advanced liver cirrhosis, compared to controls.
DOI: 10.5604/12321966.1152088
PMID 26094532 - click here to show this article in PubMed

Recommend this article to:


Related articles in IndexCopernicus™
     MMP-9 [27 related records]
     MMP-8 [3 related records]
     MMP-2 [29 related records]
     Matrix Metalloproteinases [50 related records]
     Liver Cirrhosis [166 related records]
     Alcoholic liver disease [28 related records]


Related articles

H Ho, F Lee,
The ability of 17 β-estradiol to attenuate intrah...
Background and rationale. The control of Endothelin-1 (ET-1)-mediated intrahepatic vasoconstriction in cirrhosis is beneficial for the alleviation of relevant complications. Cirrhosis is accompanied by hypogonadism and altered sex hormone status. Bes...
A Al-Qahtani, M Al-Anazi,
 PARK2 polymorphisms predict disease progress...
 Background. The protein encoded by PARK2 gene is a component of the ubiquitin-proteasome system that mediates targeting of proteins for the degradation pathway. Genetic variations at PARK2 gene were linked to various diseases including leprosy,...
S Tüzün, M Çakır,
Hepatoselüler Karsinom-Karaciğer Rezeksiyonları...
Hepatoselüler karsinom (HCC) yılda 500,000 yeni olgu ile onkolojik cerrahide önemli bir morbidite ve mortalite nedenidir. Bu yazımızda HCC’nin risk faktörleri, epidemiyolojisi, klinik seyri, tanısı ve tedavisi ile ilgili son literatür bilg...