The prevalence and distribution of Dirofilaria repens in dogs in the The prevalence and distribution of Dirofilaria repens in dogs in the Mazovian Province of Central-Eastern Poland

Aleksander Wiaczesław Demiaszkiewicz 1, Grażyna Polańczyk 2, Barbara Osińska 3, Anna Maria Pyziel 1, Izabela Kuligowska 1, Jacek Lachowicz 1, Adam Sikorski 4
1 - W. Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland
2 - ‘Megawet’, Veterinary Clinic, Milanówek, Poland
3 - Faculty of Veterinary Medicine, University of Life Sciences, Warsaw, Poland
4 - ‘Prima­Wet’, Veterinary Clinic, Warsaw, Poland
Ann Agric Environ Med
2014; 21 (4):
ICID: 1129918
Article type: Original article
The aim of this study was to determine the prevalence and distribution of canine dirofilariosis from different districts of the Mazovian Province in central-eastern Poland. 462 dogs aged from 1.5–14 years were examined for dirofilariosis. Blood samples were examined for the presence of microfilariae using the Knott method, as well as the method of Kingston and Morton, after centrifugation in haematocrit microtubes in order to determine the intensity of infection as the number of microfilariae in 60 microliters of blood. The species of microfilariae found were determined after staining on the basis of the morphological characters. Samples were also examined using Canine Heartworm Antigen Test Kit SNAP HTWM (IDEXX, USA) that allows the detection of circulating antigens of females of Dirofilaria immitis. The positive samples were examined using multiplex PCR assay for species confirmation. Microfilariae belonging to the species D. repens were found in the blood samples of dogs originating from the city of Warsaw and from 18 districts of Mazovian Province. The mean prevalence of that species observed in the province was 25.8%. The range of intensity counted with the number of microfilariae found in 60 microliters of blood amounted to between 1 – 150; median intensity 9 microfilariae. The highest prevalence, reaching 52.9% and the highest median intensity of infection reaching 28 microfilariae, was found in Radom district. The lowest prevalence, amounting to 4.2%, was registered in Grójec district; however, the lowest median intensity reaching 6 microfilariae was noted in Żyrardów district. The infected dogs had not been outside of Poland, which means that this is an autochthonous infection. Microfilariae and antigens of D. immitis were not found in any examined blood samples. Results of the presented study show that autochthonous dirofilariosis caused by nematodes D. repens commonly occurs in the area of the Mazovian Province.
DOI: 10.5604/12321966.1129918
PMID 25528905 - click here to show this article in PubMed

Recommend this article to:


Related articles in IndexCopernicus™
     Mazovian Province [0 related records]
     autochthoneus infection [0 related records]
     Dogs [2989 related records]
     Dirofilaria repens [8 related records]


Related articles

M Selwet, T Cłapa,
The Prevalence of Campylobacter s...
This study was conducted to determine the prevalence of <em>Campylobacter</em> spp. isolated from dogs’ faecal samples. From June 2012 to June 2013, a total of 210 faecal samples from pet dogs living in different kennels (n = 210) were ...
I Kaszak, M Planellas,
Pine processionary caterpillar, Thaumetopoea pityo...
Pine processionary, Thaumetopoea pityocampa Denis and Schiffermüller, 1775 is a moth that belongs to the order of insects Lepidoptera, and family Notodontidae. The larvae of pine processionary moth are the main pest of pines all over the world, but ...
B Abubakar , B Maikai ,
Prevalence of Giardia Cysts in Household Dog Faece...
Giardiasis is a major cause of diarrhoea in both humans and animals. In developing countries such as Nigeria, the disease has important Veterinary and public health consequences. This study determined the prevalence of Giardia cysts in dog faeces in ...