RESEARCH PAPER
A small scale survey of Leptospira in mammals from eastern Poland
 
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1
Department of Zoonoses, Institute of Rural Health, Lublin, Poland
 
2
Department of Parasitology, National Veterinary Research Institute, Puławy, Poland
 
3
Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Lublin, Poland
 
 
Ann Agric Environ Med. 2013;20(4):705-707
 
KEYWORDS
ABSTRACT
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland and exposed to floods by the Vistula river, and on area ‘B’, also located in eastern Poland, but not exposed to floods. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of Leptospira DNA. From 7 species of small mammals examined, the presence of Leptospira DNA was detected in 2 of them. The prevalence of positive results was greatest in Apodemus agrarius which was the mostly numerous mammal species (14 out of total 39 specimens, 35.9%). The presence of Leptospira DNA was also found in Microtus arvalis (1 out of 1 specimen, 100%), whereas the remaining 5 species (Apodemus flavicollis , Apodemus sylvaticus, Microtus agrestis, Myodes glareolus, Sorex araneus) were negative. No significant difference in the prevalence of positive findings was found between the small mammals from areas ‘A’ exposed to flooding, compared to those from area ‘B’ not exposed to flooding (20.0% vs. 30.0%, p=0.3748). The overall positivity of the examined small mammals population from areas ‘A’ and ‘B’ was 25.0%. The prevalence of dual positivity (leptospiral DNA found both in kidney and liver) was greater in the mammals from areas exposed to flooding compared to those from areas not exposed to flooding (16.7% vs. 6.7%), but this dependence was also not significant (p=0.2382).
 
REFERENCES (31)
1.
Evangelista KV, Coburn J. Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses. Future Microbiol. 2010; 5: 1413–1425.
 
2.
Adler B, de la Peña Moctezuma A. Leptospira and leptospirosis. Vet Microbiol. 2010; 140: 287–296.
 
3.
Levett PN. Leptospirosis. Clin Microbiol Rev. 2001; 14: 296–325.
 
4.
Vijayachari P, Sugunan AP, Shriram AN. Leptospirosis: an emerging global public health problem. J Biosci. 2008; 33: 557–569.
 
5.
Cruz LS, Vargas R, Lopes AA. Leptospirosis: a worldwide resurgent zoonosis and important cause of acute renal failure and death in developing nations. Ethn Dis. 2009; 19(Suppl 1): S1–37–41.
 
6.
Pappas G, Papadimitriou P, Siozopoulou V, Christou L, Akritidis N. The globalization of leptospirosis: worldwide incidence trends. Int J Infect Dis. 2008; 12: 351–357.
 
7.
Hartskeerl RA, Collares-Pereira M, Ellis WA. Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. Clin Microbiol Infect. 2011; 17: 494–501.
 
8.
Dutkiewicz J, Cisak E, Sroka J, Wójcik-Fatla A, Zając V. Biological agents as occupational hazards – selected issues. Ann Agric Environ Med. 2011; 18: 286–293.
 
9.
Hałat Z, Korczyńska A, Kasiński J: Leptospirosis outbreak in the Wrocław province in 1974. Przegl Epidemiol. 1976; 30: 492–495 (in Polish).
 
10.
Zwierz J. Leptospiroses. Edition 2. PZWL, Warsaw 1964 (in Polish).
 
11.
Wasiński B, Sroka J, Wójcik-Fatla A, Zając V, Cisak E, Knap JP, Sawczyn A, Dutkiewicz J: Seroprevalence of leptospirosis in rural populations inhabiting areas exposed and not exposed to floods in eastern Poland. Ann Agric Environ Med. 2012; 19: 285–288.
 
12.
Wasiński B, Dutkiewicz J. Leptospirosis – current risk factors connected with human activity and the environment. Ann Agric Environ Med. 2013; 20: 239–244.
 
13.
Lau CL, Smythe LD, Craig SB, Weinstein P. Climate change, flooding, urbanisation and leptospirosis: fuelling the fire? Trans R Soc Trop Med Hyg. 2010; 104: 631–638.
 
14.
Wójcik-Fatla A, Zając V, Cisak E, Sroka J, Sawczyn A, Dutkiewicz J. Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks from eastern Poland. Ann Agric Environ Med. 2012; 19: 656–659.
 
15.
Wójcik-Fatla A, Zając V, Knap JP, Sroka J, Cisak E, Sawczyn A, Dutkiewicz J. A small-scale survey of hantavirus in mammals from eastern Poland. Ann Agric Environ Med. 2013; 20: 283–286.
 
16.
Amutha R, Chaudhury P, Garg AP, Vasan P, Cheema PS, Srivastava SK. Cloning and sequence analysis of the gene encoding LipL32 of Leptospira interrogans serovar Sejroe. Vet Res Commun. 2007; 31: 513–519.
 
17.
Korczyńska A, Suchowiak J. An epidemic of marsh fever in the Wrocław province in 1971. Przegl Epidemiol. 1973; 37: 563–568 (in Polish).
 
18.
Zwierz J, Chrzanowski B, Durlakowa I, Trzankowski J. Investigations on Leptospira carriers in rodents. Med Dośw Microbiol. 1950; 2: 218–219 (in Polish).
 
19.
Konarska D. A survey of rodents and domestic animals in a focus of the epidemic of leptospirosis in the Wrocław province in 1971. Przegl Epidemiol. 1974; 38: 535–542 (in Polish).
 
20.
Zwierz J, Durlakowa I, Karmańska K, Zwierzchowski J, Łazuga K, Korczyńska A. Investigations of the fauna in the foci of leptospirosis epidemic in the Tomaszów Lubelski region. Ann UMCS 1958; Sectio D, 13 (38): 421–437 (in Polish).
 
21.
Stanko M, Prokopcáková H, Peterková J, Cisláková L. Relation between characteristics of the small mammal population and occurrence of Leptospira antibodies. Vet Med (Praha). 1997; 42: 355–360 (in Slovak).
 
22.
Kocianová E, Kozuch O, Bakoss P, Rehácek J, Kovácová E. The prevalence of small terrestrial mammals infected with tick-borne encephalitis virus and leptospirae in the foothills of the southern Bavarian forest, Germany. Appl Parasitol. 1993; 34: 283–290.
 
23.
Treml F, Nesnalová E. Serologic screening for the occurrence of Leptospira antibodies in small wild mammals. Vet Med (Praha). 1993; 38: 559–568 (in Czech).
 
24.
Adler H, Vonstein S, Deplazes P, Stieger C, Frei R. Prevalence of Leptospira spp. in various species of small mammals caught in an inner-city area in Switzerland. Epidemiol Infect. 2002; 128: 107–109.
 
25.
Koizumi N, Muto M, Yamamoto S, Baba Y, Kudo M, Tamae Y, Shimomura K, Takatori I, Iwakiri A, Ishikawa K, Soma H, Watanabe H. Investigation of reservoir animals of Leptospira in the northern part of Miyazaki Prefecture. Jpn J Infect Dis. 2008; 61: 465–468.
 
26.
Cho MK, Kee SH, Song HJ, Kim KH, Song KJ, Baek LJ, Kim HH, Oh HB, Kim YW, Chang WH. Infection rate of Leptospira interrogans in the field rodent, Apodemus agrarius, in Korea. Epidemiol Infect. 1998; 121:685–690.
 
27.
Songer JG, Chilelli CJ, Reed RE, Trautman RJ. Leptospirosis in rodents from an arid environment. Am J Vet Res. 1983; 44: 1973–1976.
 
28.
Gamage CD, Koizumi N, Muto M, Nwafor-Okoli C, Kurukurusuriya S, Rajapakse JR, Kularatne SA, Kanda K, Lee RB, Obayashi Y, Watanabe H, Tamashiro H. Prevalence and carrier status of leptospirosis in smallholder dairy cattle and peridomestic rodents in Kandy, Sri Lanka. Vector Borne Zoonotic Dis. 2011; 11:1041–1047.
 
29.
Bunnell JE, Hice CL, Watts DM, Montrueil V, Tesh RB, Vinetz JM. Detection of pathogenic Leptospira spp. infections among mammals captured in the Peruvian Amazon basin region. Am J Trop Med Hyg. 2000; 63: 255–258.
 
30.
Suepaul SM, Carrington CV, Campbell M, Borde G, Adesiyun AA. Serovars of Leptospira isolated from dogs and rodents. Epidemiol Infect. 2010; 138: 1059–1070.
 
31.
Kollars TM Jr, Phulsuksombati D, Kingnate D, Prachumsri J, Rachphaew N, Monkanna T, Gettayakamin M. Antibodies to leptospirosis in rodents from Thailand using a modified human diagnostic assay. J Med Assoc Thai. 2002; 85: 67–70.
 
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