Anti-epileptic drugs inhibit viability of synoviocytes in vitro
Jolanta Parada-Turska 1, Patrycja Nowicka-Stążka 2, Maria Majdan 3, Mirosław Jabłoński 4, Waldemar A. Turski 5, Wojciech Rzeski 6 1 - Department of Rheumatology and Connective Tissue Diseases, Medical University, Lublin, Poland 2 - Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland 3 - Department of Rheumatology and Connective Tissue Diseases, Medical University, Lublin, Poland 4 - Department of Orthopedics and Rehabilitation, Medical University, Lublin, Poland 5 - Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland; Department of Toxicology, Institute of Rural Health, Lublin, Poland 6 - Department of Medical Biology, Institute of Rural Health, Lublin, Poland; Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland Ann Agric Environ Med 2013; 20 (3): ICID: 1067442 Article type: Original article
Introduction and objective: The hyperplasia of synovial fibroblasts is considered to be essential for the evolution of joint destruction in rheumatoid arthritis (RA). Previously, we reported that anti-rheumatic drugs, both COX inhibitors and disease-modifying anti-rheumatic drugs inhibit proliferation of synoviocytes in vitro. The presented study investigates the effect of anti-epileptic drugs on the viability and proliferation of synovial fibroblasts in vitro.
Methods: Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. Cell proliferation and viability were assessed by means of BrdU assay and MTT assay, respectively. The IC50 value (the concentration of drug necessary to induce 50% inhibition) together with confidence limits was calculated.
Results: Carbamazepine inhibited proliferation of human fibroblasts and viability of HIG-82 with IC 50 values of 86 µM and 82 µM, respectively. Diphenylhydantoin, valproate and phenobarbital inhibited viability of HIG-82 cells with the IC50 values of 110, 500 and 1031 µM, respectively.
Conclusion: Based on these findings, it can be suggested that anti-epileptic drugs may have a disease-modifying effect on rheumatoid synovial proliferation.
PMID 24069867 - click here to show this article in PubMed