Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs

Valentina Virginia Ebani 1, Fabrizio Bertelloni 1, Barbara Turchi 1, Domenico Cerri 1
1 - Department of Animal Pathology, Prophylaxis and Food Hygiene. Faculty of Veterinary Medicine, University of Pisa, Italy
Ann Agric Environ Med
2013; 20 (2):
ICID: 1052333
Article type: Short communication
 
 
The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
PMID 23772578 - click here to show this article in PubMed
  FULL TEXT STATS

Recommend this article to:

Name:
E-mail:
From:
Language:


Related articles in IndexCopernicus™
     Polymerase Chain Reaction. [2 related records]
     indirect immunofluorescence antibody test [0 related records]
     hunting dogs [0 related records]
     Anaplasma phagocytophilum [3 related records]



 

Related articles

W Łuczaj, A Moniuszko,
Peroxidative metabolism of arachidonic acid in th...
Objective. The objective of the study was measurement of serum arachidonic acid level as well as the product of its peroxidation – 8-isoPGF[sub]2[/sub][sub]α[/sub], and the activity of phospholipase A[sub]2[/sub] and PAF-acetylhydrolase tha...
ABSTRACT
M Krzyżak, D Maślach,
Trends of potential years of life lost due to mai...
The aim of the study was to analyse the level and the trends of Potential Years of Life Lost due to main causes of deaths in Poland in 2002–2011, with consideration of place of residence, urban-rural. The material for the study was the number of de...
ABSTRACT
L Kapka-Skrzypczak, K Sawicki,
Cholinesterase activity in blood and pesticide pre...
Introduction. On the contrary to the adult population exposed to pesticides, mostly on occupational basis, rural children are mostly exposed to pesticides deposited in the environment. However, even this constant, distributed in time exposure to low ...
ABSTRACT