Assessment of the frequency of different Borrelia burgdorferi sensu lato species in patients with Lyme borreliosis from north-east Poland by studying preferential serologic response and DNA isolates

Sambor Grygorczuk 1, Olivier Peter 2, Maciej Kondrusik 1, Anna Moniuszko 3, Joanna Zajkowska 1, Justyna Dunaj 1, Wioletta Żukiewicz-Sobczak 4, Sławomir Pancewicz 1
1 - Department of Infectious Diseases and Neuroinfections, Medical University in Białystok, Poland
2 - Central Institute of Valais Hospital, Infectious Diseases Department, Sion, Switzerland
3 - Department of Infectious Diseases and Neuroinfections, Medical University in Białystok, Poland
4 - Department of Alergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland
Ann Agric Environ Med
2013; 20 (1):
ICID: 1041671
Article type: Original article
 
 
Introduction and objective. Several Borrelia burgdorferi sensu lato species cause Lyme borreliosis throughout Europe and their geographic distribution may influence clinical manifestations of the disease. In Poland, Lyme borreliosis presents mainly with neurologic and cutaneous symptoms, while clinically overt arthritis is rare. The presented study investigates the prevalence of B. burgdorferi s.l. genospecies in a group of patients with different clinical forms and stages of Lyme borreliosis in north-east of Poland. This has not previously been studied.
Material and methods. Preferential reactivity towards different B.burgdorferi s.l. species was investigated with a previously validated immunoblot assay in sera of 33 patients with disseminated Lyme borreliosis: 10 with neuroborreliosis, 6 with acrodermatitis chronica atrophicans and 17 with osteoarticular symptoms. Also typed were B.burgdorferi s.l. DNA isolated from the skin and synovial fluid of 7 patients with erythema migrans, acrodermatitis chronic atrophicans and arthritis.
Results. Preferential reactivity was detected in 30 out of 33 serum samples. Of these, 25 reacted preferentially with B.afzelii, 3 with B. garinii and 2 with B. burgdorferi ss. B.burgdorferi DNA was isolated from all studied samples and typed as B.afzelii in 5. In a patient with acrodermatitis chronica atrophicans studied with both methods simultaneously, B.afzelii was identified by both genotyping and serotyping.
Conclusions. Both methods gave consistent results, indicating B.afzelii as the main agent of all the clinical forms of the Lyme borreliosis in the study area.
PMID 23540208 - click here to show this article in PubMed
  FULL TEXT STATS

Recommend this article to:

Name:
E-mail:
From:
Language:


Related articles in IndexCopernicus™
     genotyping [46 related records]
     Serology [46 related records]
     etiology [240 related records]
     Borrelia afzelii [1 related records]
     Lyme borreliosis [32 related records]



 

Related articles

K Wroński, J Kaczor,
pSyChiatriC diSorderS aS a riSK faCtor for warthi...
Introduction. warthin’s tumor is a benign neoplasm of the salivary glands also known as adenolymphoma or lymphomatous papillary cystadenoma. this tumor is most commonly observed between the fifth and seventh decades of life and occurs three times...
ABSTRACT
W Narożny , J Kuczkowski ,
Congenital cholesteatomas...
Congenital cholesteatoma is a rare ear disorder. The most common presentation is a pearly and white mass, visible with an intact tympanic membrane in individuals with no previous history of era discharge, ear surgery or perforation of tympanic membra...
ABSTRACT
P Zaletel, D Sekulić,
The association between body-built and injury occu...
Objectives: This study has aimed at identifying prevalence of injury-occurrence in 24 pre-professional-ballet-dancers (females, 16–18 years of age), and identifying the associations between the body-built and prevalence of injuries. Material and Me...
ABSTRACT