Air samplings in a Campylobacter jejuni positive laying hen flock

Marwa Fawzy El Metwaly Ahmed 1, Jochen Schulz 2, Joerg Hartung 3
1 - Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Germany; Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
2 - Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Germany
3 - Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Germany
Ann Agric Environ Med
2013; 20 (1):
ICID: 1041670
Article type: Original article
 
 
The air in laying hen houses contains high concentrations of airborne bacteria. The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis®µ air Sampler were compared in terms of their efficiency in sampling aerobic mesophilic bacteria in a laying hen house. Measurements were conducted in a laying hen flock with high prevalences of C. jejuni in order to investigate if culturable cells of this organism can also be detected by the applied methods. Airborne dust was also analyzed for the presence of C. jejuni specific DNA to assess the possible occurrence of non-culturable C. jejuni in the hen house air. The numbers of mesophilic airborne bacteria ranged from 8 × 104 – 2 × 106 CFU/m-3 when sampled using AGI-30 Impingers, and from 2 × 105 – 4 × 106 CFU/m -3 when sampled using a Coriolis®µ air Sampler. The concentrations detected simultaneously by both devices correlated well (rPearson = 0.755), but the Coriolis®µ air Sampler showed a significantly higher sampling efficiency (p<0.001). Although, the within flock prevalence of C. jejuni was high during the experiments (between 70-93%), neither of the air sampling methods could detect culturable C. jejuni from the air. However, C. jejuni specific DNA was detected in 15 out of 18 airborne dust samples by mapA PCR. Based on the results, it can be concluded that airborne culturable C. jejuni were not detectable, even with an efficient air sampler, because of their low concentration. Therefore, the risk of airborne infection to poultry workers on inhaling airborne C. jejuni seems negligible. Also, the transmission of culturable C. jejuni to neighboring farms by the airborne route is unlikely. Otherwise, the detection of airborne C. jejuni specific DNA suggests that non-culturable cells could appear in the hen house air, and in future it should be verified whether sampling stress of the air sampling methods could induce the non-culturable state.
PMID 23540207 - click here to show this article in PubMed
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