Pro-inflammatory cytokines in saliva of adolescents with dental caries disease
Agnieszka Gornowicz 1, Anna Bielawska 1, Krzysztof Bielawski 2, Stanisława Zyta Grabowska 3, Anna Wójcicka 4, Magdalena Zalewska 5, Elżbieta Maciorkowska 6 1 - Department of Biotechnology, Medical University, Bialystok, Poland 2 - Department of Synthesis and Technology of Drugs, Medical University of Bialystok, Białystok, Poland 3 - Maxillofacial and Plastic Surgery Clinic, Bialystok, Poland 4 - Maxillofacial and Plastic Surgery Clinic, Bialystok, Poland 5 - Department of Public Health, University of Bialystok, Bialystok, Poland 6 - Department of Developmental Period Medicine and Paediatric Nursing, Medical University, Bialystok, Poland Ann Agric Environ Med 2012; 19 (4): ICID: 1024063 Article type: Original article
Introduction and Objective: Dental caries is an inflammatory disease with multifactorial etiology. The presented study was conducted to test the hypothesis that the elevation of salivary cytokines – interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor (TNF-a) is changed in dental caries patients. IL-6, IL-8 and TNF-a are particularly relevant to inflammation, one of the very first responses of the host to a pathological insult.
Materials and Methods: Whole saliva from 26 patients with dental caries, as well as 10 healthy persons, was investigated for the presence of IL-6, IL-8 and TNF-a by enzyme immunoassay – ELISA.
Results: The results showed that an elevation of IL-6, IL-8 and TNF-a in unstimulated whole saliva in subjects with dental caries, compared with controls, increased and was statistically significant in all cases (p <0.05). The study also show a positive correlation between TNF-a and IL-8.
Conclusions: These data suggest links between the production of tumour necrosis factor (TNF-a), interleukin-6 (IL-6), interleukin-8 (IL-8) in saliva and dental caries disease.
PMID 23311795 - click here to show this article in PubMed