Aeromonas hydrophila subsp. dhakensis – a causative agent of gastroenteritis imported into the Czech Republic

Ivo Sedláček 1, Eva Krejčí 2, Anna Andělová 2, Michaela Sedláčková 1, Iva Porazilová 2, Pavla Holochová 3
1 - Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic
2 - Institute of Public Health, Centre for Clinical Laboratories, Ostrava, Czech Republic
3 - Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic
Ann Agric Environ Med
2012; 19 (3):
ICID: 1011073
Article type: Original article
Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp. hydrophila, the faecal isolates of A. hydrophila subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp. dhakensis faecal isolates contained higher amounts of the two minor fatty acids C13:0 and C17:1 ω8c than the A. hydrophila subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis.
PMID 23020031 - click here to show this article in PubMed

Recommend this article to:


Related articles in IndexCopernicus™
     Aeromonas [7 related records]
     identification [234 related records]
     Ribotyping [44 related records]
     Fatty Acids [164 related records]
     cpn60 sequencing [0 related records]


Related articles

I Romanova, N Sеmenova,
The Composition of Fatty Acids of Pinus sylvestris...
Palmitic, linoleic and α-linolenic acids are the major fatty acids during the study. Analyzing seasonal dynamics derived fatty acids revealed that for acids with 18 carbon atoms are characterized by virtually similar dynamics during the year in all ...
A Zhelezniak, Y Katorin,
Обеспечение инвариантност...
На основе усовершенствованной математической модели выделения объекта из видео изображения, основанной на обобщенной математической ...
O Mykhailenko,
Моделювання динаміки проце...
В статті розглянуто питання моделювання динаміки процесу дроблення руди з використанням апарату ортонормованих функцій Лагерра. Викон...