Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. II. Examination of native samples from various sources

Nimfa Maria Stojek 1, Angelina Wójcik-Fatla 2, Jacek Dutkiewicz 2
1 - Department of Water and Soil Safety, Institute of Rural Health, Lublin, Poland
2 - Department of Zoonoses, Institute of Rural Health, Lublin, Poland
Ann Agric Environ Med
2012; 19 (2):
ICID: 1001813
Article type: Original article
A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions, 45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup 1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCR-positive results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella by culture, it could be regarded as an useful complementary method.
PMID 22742805 - click here to show this article in PubMed

Recommend this article to:


Related articles in IndexCopernicus™
     Legionella [23 related records]
     detection [107 related records]
     Water [1098 related records]
     Culture [1048 related records]
     PCR [362 related records]
     efficacy [173 related records]
     water supply systems [4 related records]
     well water [5 related records]


Related articles

T Ahmad, N Arshad,
Prevalence of rotavirus, adenovirus, hepatitis A v...
Viral gastroenteritis and other water-borne diseases are the most neglected areas of research in Pakistan. To determine the quality of water, 4 enteric viruses were studied from different localities of Peshawar, Pakistan. The study validates the vira...
P Maktabi, M Javaheri Baboli,
Mercury concentrations in common carp (Cyprinus ca...
The Karoun River is major source of water for warmwater fish culture industry in southwest of Iran. The aim of the present study was to investigate the distribution of mercury in tissues of marketable common carp and in bottom sediments of fish farms...
P Haller, A Jankowski,
Natural processes occurring on Earth (fires, volcanic eruptions) and human activity in the area of acquisition and processing of energy, is the cause of the continuous contaminating the atmosphere of combustion products. The combustion of fuels is an...