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RESEARCH PAPER
Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field
1
University of Life Sciences, Poznań, Poland
2
Adam Mickiewicz University, Poznań, Poland
3
ADR Technology, Poznań, Poland
Corresponding author
Agnieszka Nowak-Terpiłowska
Poznań University of Life Sciences, Wojska Polskiego 28, 60-637, Poznań, Poland
Poznań University of Life Sciences, Wojska Polskiego 28, 60-637, Poznań, Poland
Ann Agric Environ Med. 2021;28(1):163-171
KEYWORDS
metabolic activitymorphologyfibroblastscells of prostate cancerradio-frequency electromagnetic field
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Exposure to physical hazards associated with the use of machinery in agriculture and forestry: noise, vibration, dustPrevention of occupational diseases in agriculture, forestry, food industry and wood industry
ABSTRACT
Introduction:
Currently, mobile phones and Wi-Fi are the most commonly used forms of telecommunication. The popularity of mobile telecommunications has made it necessary to investigate the problem more comprehensively and cautiously assess the possible risks, because never before in history has such a substantial proportion of the population been exposed to microwaves at comparably high levels. Some studies indicate that the high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connections can have a negative effect on human health, and can cause cancer.
Objective:
The aim of the study was to investigate the influence of the radiofrquency electromagnetic field (RF-EMF) on the metaboloc activity and morphology of normal human cells (fibroblasts) and cancer cells (prostate cancer cells).
Material and methods:
The cell cultures (human fibroblasts and prostate cancer cells) were exposed to RF-EMF at the frequency of 2.5 GHz for 24, 48 and 72h. To quantify changes in cell viability, the Cell Counting Kit – 8 was used.
Results:
It was found that the RF electromagnetic field exposure caused a significant decrease in the viability of fibroblasts, and a significant increase in cancer cells. Morphological analysis did not show significant changes in both cell lines after exposure to RF-EMF.
Conclusions:
On the basis of the obtained results, the hypothesis can be formulated that a high frequency electromagnetic field can have harmful effects on human cells.
Currently, mobile phones and Wi-Fi are the most commonly used forms of telecommunication. The popularity of mobile telecommunications has made it necessary to investigate the problem more comprehensively and cautiously assess the possible risks, because never before in history has such a substantial proportion of the population been exposed to microwaves at comparably high levels. Some studies indicate that the high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connections can have a negative effect on human health, and can cause cancer.
Objective:
The aim of the study was to investigate the influence of the radiofrquency electromagnetic field (RF-EMF) on the metaboloc activity and morphology of normal human cells (fibroblasts) and cancer cells (prostate cancer cells).
Material and methods:
The cell cultures (human fibroblasts and prostate cancer cells) were exposed to RF-EMF at the frequency of 2.5 GHz for 24, 48 and 72h. To quantify changes in cell viability, the Cell Counting Kit – 8 was used.
Results:
It was found that the RF electromagnetic field exposure caused a significant decrease in the viability of fibroblasts, and a significant increase in cancer cells. Morphological analysis did not show significant changes in both cell lines after exposure to RF-EMF.
Conclusions:
On the basis of the obtained results, the hypothesis can be formulated that a high frequency electromagnetic field can have harmful effects on human cells.
ACKNOWLEDGEMENTS
The publication was co-financed within the framework of a Ministry of Science and Higher Education program as ‘Regional Initiative Excellence’ in the years 2019–2022, Project No. 005/RID/2018/19.
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